原发性肝细胞癌组织乏氧诱导因子-1α与MRP蛋白表达水平及病理诊断意义

Expression and pathological diagnosis of HIF-1α and MRP protein in primary hepatocellular carcinoma

[目的]探讨分析原发性肝细胞癌组织乏氧诱导因子-1α与MRP蛋白表达水平之间的关系,分析其表达对患者疾病的诊断意义。[方法]收集2014年2月~2016年7月来我院进行治疗的80例原发性肝细胞癌患者肝组织,同时收集患者对应癌旁正常肝组织的石蜡样本,采用免疫组织化学SP法检测所有样本中乏氧诱导因子-1α与MRP蛋白的表达情况。[结果]HIF-1α与MRP在肝癌组织中的阳性率明显高于癌旁正常肝组织(P<0.01)。肝癌组织TNM分期Ⅲ~Ⅳ期、病理分级Ⅲ~Ⅳ级、伴有门静脉癌栓、伴有淋巴结转移时,HIF-1α阳性表达率明显增高(P<0.05)。肝癌组织伴有淋巴结转移、病理分级Ⅲ~Ⅳ级时,MRP阳性表达率明显增高(P<0.05)。在肝癌组织中,HIF-1α表达与MRP表达呈正相关(r=0.531,P<0.05)。[结论]肿瘤的恶性增殖、分化、淋巴结转移的过程中,HIF-1α与MRP的过表达可能有着重要作用,二者可能具有协同作用。

[Objective]To investigate the relationship between the expression of HIF-1α and MRP protein in primary hepatocellular carcinoma,and to analyze the diagnostic value of the expression.[Methods]Collected 80 cases of primary liver tissues of patients with hepatocellular carcinoma treated in our hospital from February 2014 to July 2016,the expression of HIF-1α and MRP protein in carcinoma and adjacent tissues were detected by immunohistochemical SP method.[Results]The positive rate of HIF-1and MRP in HCC tissues was significantly higher than that in adjacent normal liver tissues（P〈0.01）.When the liver cancer TNM stage was Ⅲ-Ⅳ stage,pathological grade Ⅲ-Ⅳ,with portal vein tumor thrombus,with lymph node metastasis,the positive expression rate of HIF-1 was significantly increased（P〈0.05）.The positive expression rate of MRP was significantly higher in HCC tissues with hepatocellular carcinoma with lymph node metastasis,pathological grade Ⅲ-Ⅳ（P〈0.05）.The expression of HIF-1 was positively correlated with the expression of MRP（r=0.531,P〈0.05）in HCC tissues.[Conclusion]The overexpression of HIF-1 and MRP may play an important role in the process of malignant tumor proliferation,differentiation and lymph node metastasis,and the two may have synergistic effects.