淫羊藿苷对大鼠正畸牙齿压力区牙周组织RANKL和Wnt3a表达的影响

The Effect of Icariin on the Expression of RANKL and Wnt3a in the Pressure Zone of Periodontal Tissue During Orthodontic Tooth Movement

目的:观察不同剂量的淫羊藿苷对大鼠正畸牙齿移动时压力区牙周组织中RANKL和Wnt3a表达的影响。方法:将24只健康雄性SD大鼠随机分为4组,根据淫羊藿苷灌胃的剂量分为生理盐水组(对照组)、1 mg/kg淫羊藿苷组、3 mg/kg淫羊藿苷组、5mg/kg淫羊藿苷组(实验组),使用50 g力近中移动左侧上颌第一磨牙。通过免疫组化方法检测压力区牙周组织中RANKL和Wnt3a蛋白的表达。结果:生理盐水组上颌第一磨牙压力区牙根和牙槽骨表面粗糙,牙周膜间隙变窄,可见骨吸收陷窝和破骨细胞,不同剂量淫羊藿苷组牙周膜间隙趋于恢复正常,骨吸收陷窝出现明显减少。RANKL和Wnt3a在生理盐水组和淫羊藿苷组的压力区牙周组织中都有表达。与生理盐水组比较,不同剂量淫羊藿苷组压力区牙周组织中RANKL的表达均显著降低,Wnt3a的表达均明显增加,且RANKL的表达随淫羊藿苷剂量的增加而逐渐减少(P<0.05),Wnt3a的表达随淫羊藿苷剂量的增加明显增加(P<0.05)。结论:不同剂量淫羊藿苷能减少正畸时牙齿移动过程中压力区牙周组织中RANKL的表达,增加Wnt3a的表达,且作用与其剂量具有一定的相关性。

Objective：To investigate the effect of different dosage of icariin on the RANKL and Wnt3a expressions in the pressure side of periodontal tissue during orthodontic tooth movement （OTM）.Methods：Twenty four male SD rats were randomly divided into four groups according to the different dosage of icariin as follows：physiological saline group （control group）;1 mg/kg icariin group;3 mg/kg icariin group;5 mg/kg icariin group （experience group）.50 g force was used to move the left maxillary first molar.Immunohistochemical staining technology was used to detect the expression of RANKL and Wnt3a in the pressure side of periodontal tissue.Results：Rats in the physiological saline group showed crude surfaces of the root of tooth and alveolar bone at the pressure area of the maxillary first molar and narrowed space of the periodontium,and bone resorption pits and osteoclasts could be observed.Rats in various dosage of icariin group displayed that the space of the periodontium tended to recover normally,and significantly reduced bone resorption pits could be noted.RANKL and Wnt3a expressed on both the physiological saline and icariin groups in the pressure zone of periodontal tissue.Compared with the saline group,the expression of RANKL was significantly decreased in the pressure side of periodontal tissue in the different dosage of icariin groups,and the expression of Wnt3a were significantly increased.Furthermore,the expression of RANKL was decreased gradually with the increase of dosage （P〈0.05）.And the expression of Wnt3a was increased gradually with the increase of dosage （P〈0.05）.Conclusions：Different dosage of icariin could reduce the expressions of RANKL and increase the expression of Wnt3a in the periodontal tissue remodeling during OTM,and its effect has certain correlation with its dosage.