氟对小鼠胰岛β细胞增殖和胰岛素分泌的影响

Effects of Different Doses of Fluoride on the Function with Pancreatic Beta Cells in Mice

目的:观察不同剂量氟化钠(NaF)对体外培养的小鼠胰岛β细胞增殖活力和胰岛素分泌的影响。方法:选用小鼠胰岛β细胞株Beta-TC-6作为实验对象,分别以0、0.1、0.5、1.0、2.0、4.0、8.0、16.0 mg/LNa F干预24 h、48 h、72 h、96 h观察对β细胞形态学的影响,采用四唑蓝[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT]比色法,检测不同剂量NaF对β细胞增殖活力的影响;用酶联免疫吸附(enzyme linked immunosorbent assay,ELISA)法测定不同剂量NaF对β细胞胰岛素分泌的影响。结果:0.5 mg/L、1.0 mg/L的NaF作用72 h时,可使胰岛β细胞增殖活力和胰岛素分泌较对照组明显增强(P<0.05);≥8.0 mg/L时随着NaF剂量的增加和作用时间的延长,胰岛β细胞的增殖活力和胰岛素分泌明显减弱(P<0.05)且随着NaF剂量的增加和时间的延长,细胞生长缓慢,数量减少,不易贴壁或融合成片,多边形细胞减少,可见较多椭圆或圆形细胞。结论:NaF对胰岛β细胞的增殖和胰岛素分泌呈剂量效应关系,随着剂量的增大和时间的延长对细胞增殖活力和胰岛素分泌能力的抑制逐渐增强。

Objective：To observe the effects of different doses of sodium fluoride （NaF） on the proliferation activity and insulin secretion of mouse pancreatic islets in vitro.Methods：The mouse islet beta cell line Beta-TC-6 was used as the experimental object,to observe the effect of beta cell morphology were NaF 0,0.1,0.5,1,2,4,8,16 mg/L intervention 24 h,48 h,72 h,96 h.The effect on the proliferation activity of beta cells with different doses of NaF was detected by using the MTT method.The effect of different doses of NaF on insulin secretion of beta cells was determined by enzyme linked immunosorbent assay （ELISA）.Results：Doses of NaF 0.5 mg/L,1.0 mg/L intervented 72 h the proliferation activity and insulin secretion of pancreatic islet beta cells were significantly increased（P 0.05） compared with the control group （P〈0.05）.More than 8 mg/L with the increase of NaF dose and time of islet beta cell proliferation and insulin secretion was significantly decreased （P 〈0.05）,and with the increase of NaF dose and time,the cell growth was slow,the number was reduced,not easy to adhere to the wall or fusion into pieces,polygon cells reduced,visible more oval or round cell.Conclusion：Effects of NaF on the proliferation and insulin secretion of pancreatic beta cells in a dose response relationship,the inhibition of cell proliferation and insulin secretion gradually increased with the increase of dose and time.