平喘颗粒的药效学研究

Experimental Study on Pharmacodynamics of Pingchuan Granule

目的:研究平喘颗粒的药效学作用。方法:小鼠随机分为空白组(或/和)模型组,喘嗽宁组(0.7 g/kg)、平喘颗粒组(31.2 g/kg)、平喘颗粒组(15.6 g/kg)、平喘颗粒组(7.8 g/kg)。采用酚红外排,氨水引咳,二甲苯致小鼠耳廓肿胀,琼脂导致小鼠肉芽肿炎症,小鼠负重游泳,小鼠耐常压缺氧,小鼠巨噬细胞吞噬实验观察平喘颗粒的祛痰,镇咳,抗炎,抗疲劳,抗乏氧和提高免疫力作用。大鼠随机分为空白组、模型组、喘嗽宁组(0.486 g/kg)、平喘颗粒组(21.6 g/kg)、平喘颗粒组(10.8 g/kg)、平喘颗粒组(5.4 g/kg),给予卵蛋白激发哮喘,观察平喘颗粒的平喘作用;豚鼠随机分为模型组、喘嗽宁组(0.419 g/kg)、平喘颗粒组(18.6 g/kg)、平喘颗粒组(9.3 g/kg)、平喘颗粒组(4.65 g/kg),喷入磷酸组胺和氯化乙酰胆碱,观察平喘颗粒的平喘作用。结果:平喘颗粒(31.2 g/kg、15.6 g/kg、7.8 g/kg)可显著提高小鼠酚红的排出量(P<0.05),延长氨水致小鼠咳嗽的潜伏期(P<0.05),减少氨水所致小鼠咳嗽次数(P<0.05),减少二甲苯致小鼠耳廓肿胀程度(P<0.05),减少琼脂导致小鼠肉芽肿重量(P<0.01),提高小鼠负重游泳时间(P<0.05),提高小鼠耐常压缺氧时间(P<0.05),可显著提高小鼠巨噬细胞吞噬能力(P<0.05)。平喘颗粒(21.6 g/kg、10.8 g/kg、5.4 g/kg)延长卵蛋白致大鼠哮喘发作的潜伏期(P<0.05);平喘颗粒(18.6 g/kg、9.3 g/kg、4.65 g/kg)延长磷酸组胺和氯化乙酰胆碱致豚鼠哮喘发作的潜伏期(P<0.05)。结论:平喘颗粒具有较好的镇咳,祛痰,抗急、慢性炎症,抗疲劳,抗乏氧,提高免疫力,平喘的作用。

Objective：To study the pharmacodynamics of Pingchuan granule.Methods：Mice were randomly divided into control group（or/and model group）,Chuansuning group（0.7 g/kg） and three dosage groups of Pingchuan granule（31.2 g/kg,15.6 g/kg,7.8 g/kg）.To observe the activities of Pingchuan granule on antitussive,anti-inflammatory,anti-fatigue,improving immunity,the following experiments were carried out：removing phlegm with phenol red,cough induced by ammonia,Xylene-induced mouse ear edema,chronic granuloma induced by agar,weight loading swimming test,beating hypoxia at normal pressure test,peritoneal macrophage experiment.Rats were randomly divided into six groups：control group model group））,Chuansuning group （0.486 g/kg） and three dosage groups of Pingchuan granule （21.6 g/kg,10.8 g/kg,5.4 g/kg）.To study the antiasthma effect of Pingchuan granule,the asthma latent period,which induced by OVA in rats,were observed.Guinea pigs were also randomly divided into five groups：model group,Chuansuning group （0.419 g/kg） and three dosage groups of Pingchuan granule （18.6 g/kg,9.3 g/kg,4.65 g/kg）.To study the antiasthma effect of Pingchuan granule,the asthma latent period,which induced by acetylcholine and histamine in guinea pigs,were observed.Results：Pingchuan granule （31.2 g/kg,15.6 g/kg,7.8 g/kg） could increase the phenol red excretion of respiratory tract in mice（P〈0.05）,reduce the frequency of cough induced by ammonia and prolong the latent period of cough （P〈0.05）,inhibit the mice auricle swelling by xylene（P〈0.05）and chronic granuloma by agar（P〈0.01）,prolong the duration of weight loading swimming test and hypoxia at normal pressure test improved the phagocytic index of macrophage.Pingchuan granule （21.6 g/kg,10.8 g/kg,5.4 g/kg） could prolong the asthma latent period induced by OVA in rats;Pingchuan granule （21.6 g/kg,10.8 g/kg,5.4 g/kg） could prolong the asthma latent period induced by acetylcholine and histamine in guinea pigs.Conclusions：Pingchuan granule has activities of antitussive,anti-inflammatory,anti-fatigue,improving immunity and antiasthmatic.