摘要
目的:研究长链非编码RNA(long non-coding RNA,lnc RNA)uc.299在P19细胞分化及斑马鱼心脏发育中的作用,探讨其与心脏发育之间的关系。方法:体外实验中,通过sh RNA沉默uc.299后,检测对P19分化的影响;在体实验中,对斑马鱼受精卵显微注射uc.299反义寡核苷酸,观察对斑马鱼心脏发育的影响。结果:与对照组相比,沉默uc.299对P19细胞分化有明显的抑制作用,心肌细胞跳动频率降低,各心肌标志物表达水平有明显下调。斑马鱼实验表明,沉默uc.299可造成斑马鱼心脏明显水肿。结论:沉默uc.299可影响P19分化和斑马鱼心脏发育,表明uc.299对维持心脏正常发育具有一定作用。
Objective： To investigate the function of lnc RNA uc.299 in the heart development of zebra fish and differentiation of P19 cells. Methods： In in vitro experiment, the effect of uc.299 knockdown on P19 cells differenctiation were detected; and in vivo, after macroinjecting the zygotes zebra fish with uc.299 antisense oligonucleotide, the cardic development of the zebra fish was observed. Results： The differentiation and related cardiac biomarkers of P19 are reduced as well as the heart development of zebrafish post Knockdown of uc.299. Conclusion： The differentiation and development are influenced post knockdown of uc.299. It indicates that uc.299 may play a role in maintaining heart development.
出处
《临床与病理杂志》
2017年第6期1111-1116,共6页
Journal of Clinical and Pathological Research
基金
国家自然科学基金(81601333)~~
