miR-15a-5p和miR-16-5p在骨肉瘤组织中的表达分析

Expression of miR-15a-5p and miR-16-5p in Osteosarcoma

目的:使用microRNAs基因芯片及实时定量PCR法测定骨肉瘤组织中miR-15a-5p和miR-16-5p的相对表达含量,并与瘤旁组织对比,分析骨肉瘤细胞内miR-15a-5p和miR-16-5p的表达变化。方法:选取34例骨肉瘤组织蜡块样本,使用microRNAs基因芯片观察miR-15a-5p和miR-16-5p在骨肉瘤和瘤旁组织内的表达差异;实时定量PCR法测定骨肉瘤组织和瘤旁组织中miR-15a-5p和miR-16-5p的相对表达含量,并将两种结果对比分析。结果:microRNAs基因芯片结果显示,在骨肉瘤组织中,miR-15a-5p在肿瘤中的表达较瘤旁组织低1.79倍,miR-16-5p较瘤旁组织低1.62倍。实时定量PCR实验结果表明,miR-15a-5p和miR-16-5p表达较瘤旁组织降低,差异有统计学意义(P<0.05)。经过统计学计算,miR-15a-5p在肿瘤中的表达较瘤旁组织低3.14倍,miR-16-5p较瘤旁组织低5.65倍。结论:在骨肉瘤中,miR-15a-5p和miR-16-5p表达含量降低,提示这两种microRNAs在骨肉瘤中可能做为抑癌因子存在。

Objective：Use microRNA microarray chip and real time quantitative PCR method to analysis the relative expression levels of miR-15a-5p and miR-16-5p,then compare the difference between osteosarcoma paraffin block samples and peritumoral tissue.Methods：34 cases of osteosarcoma paraffin block samples were chosen,microRNAs microarray chip and real time quantitative PCR method was used to measure the relative expression levels of miR-15a-5p and miR-16-5p.Results：microRNAs microarray chip results showed that the expression level ofmiR-15a-5p （miR-16-5p） in tumor tissue was 1.79 （1.62） times lower than the adjacent tissue.Real time quantitative PCR results showed that compare with tumor adjaccnt tissues,the expression level of miR-15a-5p and miR-16-5p was low in osteosarcoma paraffin block samples.The results were considered significant through statistical analysis （P＜0.05）.Further analysis results showed that the expression level of miR-15a-5p （miR-16-5p） in tumor tissue was 3.14 （5.65） times lower than the adjacent tissue.Conclusions：miR-15a-5p and miR-16-5p was low expressed in osteosarcoma cells,which suggest that these two microRNAs could be act as oncogene.