Osterix通过上调LOX的表达促进人乳腺癌细胞的迁移和侵袭

Osterix promotes the migration and invasion of human breast cancer cells by up-regulating LOX expression

目的:探讨Osterix(Osx)对乳腺癌细胞MDA-MB-231迁移和侵袭能力的影响及其潜在机制。方法:采用荧光实时定量PCR(Real-time PCR)及Western blot技术检测Osx过表达及敲低稳转细胞(231-OE6、231-OEC、231-KD2、231-KDC)中赖氨酰氧化酶(lysyl oxidase,LOX)的表达水平;转染LOX si RNA及其对照至Osx过表达稳转细胞(231-OE6)中,Transwell检测细胞的迁移及侵袭能力;同时转染LOX过表达质粒及其对照至Osx敲低稳转细胞(231-KD2)中,Transwell检测细胞的迁移及侵袭能力。结果:与各自对照相比,LOX在231-OE6中表达显著升高,在231-KD2中表达显著降低(P<0.05);降低LOX的表达能够显著削弱231-OE6细胞的迁移和侵袭能力,增加LOX的表达能够显著提高231-KD2细胞的迁移和侵袭能力,差异有统计学意义(P<0.05)。结论:在乳腺癌细胞中Osx能够上调LOX的表达,且Osx通过上调LOX的表达而促进乳腺癌细胞的迁移和侵袭。

Objective：To explore the roles and the potential underlying molecular mechanisms of Osterix（Osx） on the migration and invasion of breast cancer cell line MDA-MB-231. Methods： Real-time PCR and Western blot assay were performed to detect the expression levels of lysyl oxidase（LOX） in Osx stably transfected or knocked down cells as well as in their corresponding control cells（231-OE6, 231-OEC, 231-KD2, and 231-KDC）. The migration and invasion of 231-OE6 cells transfected with LOX si RNA and231-KD2 cells transfected with LOX expression plasmids were detected by Transwell assays. Results： Compared to their corresponding control cells, the expression of LOX was increased in 231-OE6 cells, and decreased in 231-KD2 cells（P 〈0.05）. Knockdown of LOX in 231-OE6 cells attenuated the increased migration and invasion. Conversely, overexpression of LOX in 231-KD2 cells rescued the decreased migration and invasion（P 〈0.05）. Conclusion： The expression of LOX is up-regulated by Osx in human breast cancer cells, and it promotes the migration and invasion of breast cancer cells.