摘要
目的:对毛茛科乌头属植物滇北乌头的块根进行化学成分研究。方法:滇北乌头的干燥块根粉碎后用甲醇渗漉提取,回收溶剂所得浸膏用1.5%HCl溶解,乙酸乙酯萃取,酸水溶液用5% NaOH调至pH为9,乙酸乙酯萃取,得总生物碱粗提物。粗提物用各种柱色谱进行分离纯化,经光谱数据(~1H-NMR,^(13)C-NMR,MS)进行结构鉴定。结果:分离鉴定18个二萜生物碱,分别为:14-O-acetylsachaconitine(1),franchetine(2),crassicaudine(3),indaconoitine(4),14-benzoyl chasmanine(5),14-Oacetyltalatisamine(6),talatisamine(7),chasmannine(8),crassicauline A(9),bikhaconine(10),13,15-dideoxyaconitine(11),crassicautine(12),kongboensine(13),liljestrandisine(14),ludaconitine(15),8-deacetyl-yunaconitine(16),yunaconitine(17),ouvrardiantine(18)。结论:首次对滇北乌头进行化学成分研究,共分离鉴定18个二萜生物碱。
Objective: To study the chemical constituents in the roots of Aconitum iochanicum Ulbr. Methods: The air-dried roots of A. iochanicum were powdered and extracted by methanol with a percolation method. After removing the solvent under reduced pressure, the crude extract was dissolved in 1.5% HCl solution, and then extracted by ecetic ether. The acidic solution was basified to pH 9.0 by NaOH (5%) and extracted with ethyl acetate to obtain crude alkaloidal extract after the removal of ethyl acetate. The com- pounds were isolated and purified by column chromatography and identified based on spectral analysis (^1H-NMR, ^13C-NMR and MS). Results: Totally 18 compounds were isolated from A. iochanicum and characterized as 14-O-acetylsachaconitine (1), franchetine (2) , crassicaudine ( 3 ) , indaconoitine ( 4 ) , 14-benzoyl chasmanine ( 5 ) , 14-O-acetyhalatisamine (6) , talatisamine ( 7 ) , chasmannine (8), crassicauline A (9), bikhaconine (10), 13,15-dideoxyaconitine (11), crassicautine (12), kongboensine (13), liljes- trandisine ( 14), ludaconitine ( 15 ), 8-deacetyl-yunaconitine ( 16), yunaconitine (17) and ouvrardiantine ( 18 ). Conclusion: It' s the first time to study the chemical constituents of A. iochanicum, and 18 diterpenoid alkaloids are isolated.
出处
《中国药师》
CAS
2017年第7期1158-1163,共6页
China Pharmacist
基金
国家自然科学基金项目(编号:21362045)
关键词
毛茛科
滇北乌头
二萜生物碱
Ranunculaceae
Aconitum iochanicum
Diterpenoid alkaloids