白假丝酵母菌阴道炎小鼠模型的构建

Establishment of Candida Albicans Vaginitis Model in Mice

目的:构建白假丝酵母菌阴道炎小鼠模型,探索最佳成模条件,以提供经济实用的白假丝醇母菌性阴道炎动物模型。方法:采用昆明雌性小鼠,预先连续灌胃给予低、中、高剂量(0.001 5,0.015,0.15 mg/10 g)的戊酸雌二醇7 d后接种白假丝酵母菌建立阴道炎模型,并进行阴道灌洗液真菌载量和阴道组织病理观察。结果:戊酸雌二醇低、中、高剂量组阴道灌洗液的菌落计数分别为124.67±19.01、217.67±22.50、282.00±27.87,高剂量组与中、低剂量组比较差异有统计学意义(P<0.05或P<0.01)。HE染色和PAS染色结果显示,戊酸雌二醇高剂量组有较多多形核粒细胞(PMNs)浸润及紫红色线状菌丝。结论:戊酸雌二醇高剂量组造模方式为昆明小鼠白假丝酵母菌阴道炎模型的最佳造模条件。

Objective： To establish Candida albicans vaginitis in mice and explore the best modeling conditions to provide an economical and practical animal model of Candida albicans vaginitis. Methods： After given estradiol valerate respectively at low, medium and high dose （0.001 5, 0.015,0.15 mg/10 g） by gavage for continual 7 days, Kunming female mice were inoculated Candida albicans to establish the vaginitis model. The amount of Candida albicans in vaginal lavage fluid was analyzed and the vaginal pathological changes were observed. Results： The colony count in vaginal lavage with estradiol valerate at low, medium and high dose was 124.67± 19.01, 217.67 ±22.50 and 282.00±27.87, respectively, and compared with that in the high dose group, there were significant differences in the medium dose group and low dose group （P 〈 0.05 or P 〈 0.01 ）. HE staining and PAS staining showed that the high dose group had more polymorphonuclear neutrophils （PMNs） infiltration and purple red line hypha. Conclusion： Estradiol valerate at high dose is the best modeling condition for Candida albicans vaginitis in mice.