HPLC-DAD法同时测定复方银翘氨酚维C片中5种组分的含量

Simultaneous Determination of Five Components in Compound Paracetamol Vitamin C Yinqiao Tablets by HPLC-DAD

目的:同时测定复方银翘氨酚维C片中连翘苷、绿原酸、马来酸氯苯那敏、维生素C和对乙酰氨基酚5种组分的含量。方法:采用Waters Xselect C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1 mol·L^(-1)磷酸二氢钠(pH 3.0),梯度洗脱,流速为1.0 ml·min^(-1),检测波长230 nm,柱温35℃。结果:维生素C、对乙酰氨基酚、绿原酸、马来酸氯苯那敏、连翘苷分别在0.500~200.200,0.499~199.600,0.501~200.100,0.502~200.900,0.488~195.400μg·ml^(-1)范围内呈良好线性关系(r>0.999),平均回收率分别为99.3%(RSD=1.5%)、97.8%(RSD=1.7%)、98.5%(RSD=1.1%)、97.1%(RSD=1.7%)、99.8%(RSD=1.2%)(n=9)。结论:该方法操作简单、灵敏度高,可用于复方氨酚维C银翘片的质量控制。

Objective： To simultaneously detect five components （phillyrin, chlorogenic acid, chlorpheniramine maleate, vitamin C and acetaminophen） in compound paracetamol vitamin C Yinqiao tablets. Methods： Using a Waters Xselect C18 column（250 mm × 4.6 mm, 5μm）, the mobile phase consisted of acetonitril-0.1 mol·L^-1 NaH2PO4 （ pH 3.0） with gradient elution at a flow rate of 1.0 ml·min^-1. The detection wavelength was 230 nm and the column temperature was 35℃. Results： The linear range of vitamin C, acetaminophen, chlorogenic acid, chlorphenamine maleate and phillyrin was 0. 500-200. 200, 0.499-199. 600, 0. 501-200. 100, 0. 502-200. 900 and 0. 488-195. 400μg·ml^-1 （ r 〉 0. 999）, respectively. The average recovery was 99.3% （RSD = 1.5% ）, 97.8% （RSD = 1.7% ）, 98.5% （RSD = 1.1% ）, 97.1% （RSD = 1.7% ） and 99.8% （RSD = 1.2% ） （ n = 9）, respectively. Conclusion： The simple and sensitive method can be applied in the quality control of compound paracetamol vitamin C Yinqiao tablets.