UTP23基因在上皮性卵巢癌紫杉醇耐药细胞中的表达及对紫杉醇化疗耐药的影响

Expression of UTP23 gene in epithelial ovarian cancer paclitaxel resistant cells and its effect on paclitaxel resistance to chemotherapy

目的:探讨UTP23基因在上皮性卵巢癌紫杉醇耐药细胞中的表达及对紫杉醇化疗耐药的影响。方法:采用逆转录聚合酶链式反应(RTPCR)和蛋白免疫印迹(Western blot)法检测上皮性卵巢癌紫杉醇耐药细胞中UTP23、P-糖蛋白(Pgp)、多药耐药基因(MDR1)的mRNA表达水平,使用特异性UTP23-siRNA转染上皮性卵巢癌紫杉醇耐药细胞,使用RT-PCR和Western blot法检测转染后UTP23、P-gp、MDR1基因的表达,使用MTT法检测UTP23-siRNA对上皮性卵巢癌紫杉醇耐药细胞生长的影响及耐药性。结果:耐药组UTP23基因mRNA表达水平明显低于敏感组(P<0.05),耐药组P-gp、MDR1基因mRNA表达水平明显高于敏感组(P<0.05);转染组与未转染组、阴性对照组相比较,UTP23 mRNA水平明显升高(P<0.05),未转染组与阴性对照组相比较,UTP23 mRNA水平和蛋白水平差异无统计学意义(P>0.05);转染组与未转染组、阴性对照组相比较,P-gp、MDR1 mRNA表达水平明显下降,差异有统计学意义(P<0.05),未转染组与阴性对照组相比较,P-gp、MDR1 mRNA表达水平无明显变化,差异无统计学意义(P>0.05);紫杉醇对UTP23-siRNA转染后上皮性卵巢癌耐药细胞的IC50浓度明显高于未转染组和阴性对照组(P<0.05)。结论:UTP23基因在上皮性卵巢癌紫杉醇耐药细胞中低表达,其可能通过间接调控MDR1及P-gp的表达而参与上皮性卵巢癌紫杉醇细胞对紫杉醇的耐药。

AIM： To investigate the expression of UTP23 gene in epithelial ovarian cancer paclitaxel resistant cells and its effect on the resistance to paclitaxel chemotherapy. METHODS： The reverse transcriptase polymerase chain reaction（ RT-PCR）and Western blot were used to detect the expression level of UTP23,P-gp,MDR1 mRNA in epithelial ovarian cancer paclitaxel resistant cells; specific UTP23-siRNA was used to transfect epithelial ovarian cancer paclitaxel resistant cells; the expression of UTP23 gene after transfection was detected by RTPCR and Western blot method; the effect of UTP23-iRNA on the growth of epithelial ovarian cancer paclitaxel resistant cells and drug resistance were detected by MTT method. RESULTS：The expression level of UTP23 gene mRNA in the resistant group was significantly lower than that in the sensitive group（ P 0. 05）; the expression levels of P-gp mRNA and MDR1 mRNA in the resistant group were significantly higher than those in the sensitive group（P 0. 05）; compared with the non transfection group and the negative control group,the levels of UTP23 mRNA in the transfection group were significantly increased（ P 0. 05）; compared with the negative control group, there was no significant difference of the level of UTP23 mRNA and protein level in the non transfection group（ P 0. 05）;compared with the non transfection group and the negative control group,the expression levels of P-gp mRNA and MDR1 mRNA were significantly decreased in the transfection group（ P 0. 05）; compared with the negative control group,there was no significant difference of the expression level of P-gp and MDR1 in mRNA in the transfection group（ P 0. 05）; Paclitaxel on UTP23-siRNA after transfection,the concentration of IC50 in epithelial ovarian cancer resistant cell in the transfection group was significantly higher than those of the non transfection group and negative control group（ P 0. 05）.CONCLUSION： The expression level of UTP23 gene in epithelial ovarian cancer paclitaxel resistant cells is low; it may be involved in the resistance of paclitaxel cells to paclitaxel in epithelial ovarian cancer by indirectly regulating the expression of MDR1 and P-gp.