高效谷子原生质体培养体系的建立

An Efficient Protoplast Culture System for Millet (Setaria italica L.)

通过变换培养基成份,调控谷子愈伤组织状态,从不能直接用于原生质体培养的致密型愈伤组织中,选出了生长旺盛的松软型愈伤组织,将其用于原生质体培养,获得了高频率的细胞分裂;又从不能直接用于植株分化的松软型再生愈伤组织中,选出了致密型愈伤组织并诱导分化出大量原生质体再生植株.

Mature embryos or inflorescences of 113 genotypes of millet(Setaria italica L.) were screened for callus initiation, in which 89% of genotypes produced compact calli. In the preliminary experiment, it was found that the compact calli derived from mature embryo or inflorescence culture could not be directly used for protoplast isolation and culture. By altering the medium composition, the vigorous loose calli were selected from compact calli of the cultivar Jigu No 11 and were successfully used for protoplast isolation and culture which resulted in a high frequency of cell division (33.5% at day 14) . Since protoplast-derived calli were loose in appearence which could not differentiate, the medium composition was again altered by which the compact calli were selected from loose calli and 129 plantlets regenerated. The regenerated plants were transplanted into experimental plot. Among them 101 plants grew to maturity.