基因型和培养基对小麦幼胚离体培养的影响

Effect of Genotypes and Mediums on Wheat Immature Embryos in vitro Culture

本文对10个基因型的小麦幼胚在6种培养基上的离体培养反应进行了分析.结果表明,以MS培养基为基础,把无机盐的浓度加倍或附加氯化钠和氧化钾各1克/升可使愈伤组织诱导率降低,而以Dicamba(4毫克/升)代替2,4-D(2毫克/升)则使愈伤组织诱导率提高.所有供试基因型的幼胚愈伤组织诱导率都在95%以上,愈伤组织分化率变动在73.7～92.5%之间,平均为84.6%.基因型是影响小麦幼胚愈伤组织诱导和分化的一个极为重要的因素.

The response of immature embryos of 10 wheat genotypes to in vitro cultures on 6kinds of medium was analysed in this paper. The result indicated that doubling the concentration of inorganic solts or adding 1g/1 of NaCl and KCl each on the basis of MS medium cut down the callus induction frequency, while substituting dicamba (4mg/l) for 2,4-D (2mg/l) increased the callus induction frequency. The callus induction frequency of 10 wheat genotypes tested were more than 95%. Callus regeneration frequency varied from 73.7%to 92.5%, and the average was 84.6%. Genotype is a very important factor effecting callus induction and callus regeneration of wheat immature embryos.