中老年人睡眠与外周血白细胞端粒长度的关联性分析

Association between sleep and leukocyte telomere length in middle-aged and older adults

目的探索中老年人群睡眠与外周血白细胞端粒长度的关联性，为提倡健康睡眠延缓衰老提供科学依据。方法使用匹兹堡睡眠质量指数调查表（PSQI）及一般情况调查表，对176名中老年人进行睡眠质量状况调查；采用荧光定量PCR法测量外周血白细胞端粒相对长度，并进行相关和回归分析。结果外周血白细胞端粒平均长度为（0．995±0．23）T／S，年龄与端粒长度呈负相关（r=-0．241，P=0．003）；睡眠与年龄有关，随着年龄的增加，睡眠质量更差（r=-0．230，P〈0．01）、入睡时间更长（r=0．227，P〈0．01）、睡眠时间更短（r=-0．486，P〈0．01）、睡眠效率更差（r=-0．226，P〈0．01）；校正性别、年龄、婚姻、学历、家庭月收入、居住形式、吸烟、饮酒、体育锻炼、基础疾病因素的影响后，线性回归分析显示睡眠时间（β=0．057，P〈0．01）、入睡时间（β=-0．046，P〈0．01）、睡眠质量（β=0．086，P〈0．01）是外周血白细胞端粒长度的独立影响因素，表明睡眠时间越长、入睡时间越短、睡眠质量越好，端粒长度越长。结论睡眠是影响中老年人群端粒长度的因素，优良的睡眠可能通过减缓端粒长度的缩短延缓衰老，鼓励在社区进行健康睡眠知识的宣教活动，提高睡眠质量。

Objective To understand the association between peripheral leukocytes telomere length （TL） and sleep in middle-aged and old adults. Methods A total of 176 middle-aged and old adults were investigated by using the Pittsburgh Sleep Quality Index and questionnaire. TL was measured by fluorescence quantitative PCR. The correlation and regression analysis between sleep and telomere length was performed. Results TL had a mean T/S ratio of 0.995 ± 0.23. There was a negative correlation between TL and age （r=-0.241, P=0.003）. With increasing age, sleep quality became worse （r=-0.230, P〈0.01）, the time to fall asleep became longer （r=0.227, P〈0.01）, sleep duration was shorter （r=-0.486, P〈0.01）, sleep efficiency became worse （r=-0.226, P〈0.01）. After controlling for the effects of gender, age, marital status, income level, residence, smoking, drinking, physical exercise and disease status, multiple linear regression analysis indicated that sleep quality （13= 0.057, P〈0.01）, time to fall asleep （/3=-0.046, P〈0.01）, sleep duration （fl=0.086, P〈0.01） were independent influencing factors of telomere length, suggesting that the people who had better sleep quality , the shorter time to fall asleep, the longer sleep time would have longer telomere length. Conclusions Sleep is a relevant factor affecting TL in middle-aged and elderly population. Good sleep may delay aging by slowing TL. We encourage to conduct health education about the importance of sleep quality in community.