舌鳞癌患者外周血Th17细胞及其细胞因子变化的临床意义

Clinical significance of peripheral blood Th17 cells and their cytokines in tongue squamous cell carcinoma

目的探讨舌鳞癌患者外周血辅助性T细胞17(Th17)及其细胞因子变化的临床意义。方法以该院2013年1月至2016年1月收治的66例舌鳞癌患者为研究对象,同期行健康体检的42例志愿者为对照。采集所有研究对象外周血,流式细胞术检测外周血Th17细胞比例,ELISA法检测白细胞介素(IL)-17、转化生长因子-β(TGF-β)和IL-6因子水平。结果观察组和对照组外周血Th17细胞比例、IL-17、TGF-β、IL-6分别为(1.46±0.41)%、(0.31±0.12)%,(123.36±21.20)pg/mL、(20.76±8.95)pg/mL,(215.80±21.52)pg/mL、(26.90±10.41)pg/mL,(17.32±8.02)pg/mL、(5.85±1.49)pg/mL,差异有统计学意义(P<0.05)。Th17细胞比例、IL-17、TGF-β和IL-6水平随着舌鳞癌临床分期的增加而升高(P<0.01)。TGF-β与IL-17水平呈正相关(r=0.626,P=0.021),IL-6水平与Th17细胞比例和IL-17均呈正相关(r=0.626、0.597,P=0.021、0.034)。结论Th17细胞和IL-17表达的增加在舌鳞癌的发生、发展中发挥着重要作用,Th17细胞和IL-17因子可成为抗肿瘤治疗的重要靶点。

Objective To investigate the clinical significance of peripheral blood Th17 cells and their cytokines in the patients with tongue squamous celt carcinoma. Methods Sixty-six cases of t0ngue squamous cell carcinoma in our hospital from January 2013 to January 2016 served as the research subjects and contemporaneous 42 volunteers undergoing healthy physical examination were selected as the control group. Peripheral blood was collected in all subjects. The proportion of peripheral blood Thl7 cells was detected by flow cytometry. The IL-17, TGF-β and IL-6 cytokine levels were detected by ELISA. Results The proportion of periph- eral blood Thl7 cells, IL-17, TGF-9 and IL-6 in the observation group and the control group were （1.46 ± 0.41 ）% vs. （0. 31 ± 0.12）%,（123.36±21.20）pg/mL vs. （20.76±8.95）pg/mL,（215.80±21.52）pg/mLvs. （ 26. 90 ±10. 41） pg/mL , （17. 32 ± 8.02）pg/mL vs. （5.85 ± 1. 49）pg/mL respectively, the differences were statistically significant（P〈0. 05）. The proportion of pe- ripheral blood Th17 cells,IL-17,TGF-β and IL-6 levels were increased with the increase of tongue squamous cell carcinoma clinical stage （P〈0.01）. The TGF-β level was positively correlated with the IL-17 level （r=0. 626, P=0. 021）, the IL-6 level was posi- tively correlated with the proportion of Th17 ceils and IL-17 （r=0. 626,0. 597, P=0. 021.0. 034）. Conclusion The increase of Th17 cells and IL-17 expression plays an important role in the development and progression of tongue squamous cell carcinoma, Thl7 cells and IL-17 cytokine can become the important target spots of anti-tumor therapy.