CD68^+和CD163^+巨噬细胞在口腔鳞状细胞癌中的浸润及作用

Infiltration and effect of CD68^+ and CD163^+ macrophages in oral squamous cell carcinoma

目的了解CD68^+、CD163^+巨噬细胞的浸润数量与口腔鳞状细胞癌(OSCC)临床特征的相关性,探讨CD68^+、CD163^+巨噬细胞在OSCC中的作用及意义。方法选取贵州医科大学附属口腔医院和贵阳市口腔医院2013-2016年手术切除的,经术后病理确诊,且有完整临床资料的OSCC患者42例,另设12例口腔颌面部正常组织作对照。应用免疫组织化学法检测CD68^+、CD163^+巨噬细胞在OSCC中的浸润数量,并分析其与OSCC临床特征的相关性。结果 OSCC中CD68^+、CD163^+巨噬细胞的浸润数量明显高于对照组(P<0.05);CD68^+、CD163^+巨噬细胞在中低分化的浸润数量明显高于高分化的浸润数量(P<0.05);CD68^+巨噬细胞在有淋巴结转移的浸润数量明显低于无淋巴结转移的数量(P<0.05),在有淋巴结转移中CD163^+巨噬细胞的浸润数量明显高于CD68^+(P<0.05)。CD68^+和CD163^+巨噬细胞在OSCC中的浸润数量呈正相关(r=0.48,P=0.00)。结论肿瘤相关巨噬细胞(TAMs)、M2型巨噬细胞对OSCC具有一定的作用,TAMs可能抑制OSCC的淋巴结转移,而M2型巨噬细胞可能促进OSCC的淋巴结转移。

Objective To investigate the correlation between the number of CD68＋ and CD163＋ macrophages infiltration and the clinical characteristics of oral squamous cell carcinoma （OSCC）, and to investigate the role and significance of CD163＋ and CD68＋ macrophages in OSCC. Methods Forty-two cases of OSCC reseeted by operation and diagnosed by postoperative pathology with intact clinical data in our hospitals during 2013 - 2016 were selected. Other 12 cases of oral mamaxillofacial normal tissue served as controls. The number of CD68＋ and CD163＋ macrophages infiltration in OSCC was detected by immunohistochemistry. Its correlation with clinical characteristics was analyzed. Results The number of CD68＋ and CD163＋ macrophages infiltration in OSCC was significantly higher than that in the control group （P〈0.05） ;the infiltration quantity of CD68＋ and CD163 ＋ macropha- ges in poor differentiation and moderate differentiation was significantly higher than that in high differentiation（P〈0.05）. The number of CD68＋ macrophages in lymph node metastasis was significantly lower than that without lymph node metastasis （P〈 0.05）. The number of CD163＋ macrophages in the lymph node metastasis was significantly higher than that of CD68＋ （P〈0.05）. The number of CD68＋ and CD163＋ macrophages had positive correlation in OSCC （r=0.48,P=0.00）. Conclusion Tumor-associ- ated macrophages （TAMs） and M2 macrophages may play an important role in OSCC,TAMs may inhibits lymph node metastasis in OSCC,while M2 marcophages may promote the lymphatic matastasis of OSCC.