摘要
目的探讨mi R-423、mi R-205和mi R-1307单核苷酸多态性与宫颈癌的相关性。方法以病例-对照研究的方法收集104例宫颈癌及186例骨折、高血压等同期非肿瘤患者为对照,采用PCR-限制性酶切片段长度多态性技术对mi R-423 rs6505162位点进行基因型分析;采用PCR和多重连接酶检测反应技术对mi R-205 rs3842530和mi R-1307 rs7911488基因多态性进行分析。结果 rs6505162 CC、rs6505162 AC和rs6505162AA基因型在宫颈癌组中分布为64.4%、31.7%和3.8%,对照组分布为66.1%、30.1%和3.8%;rs3842530-/-、-/insert和inser/inser基因型在宫颈癌组中分布为56.7%、34.6%和8.7%,对照组分布为52.7%、36.0%和7.0%;rs7911488 AA、AG和GG基因型在宫颈癌组中分布为51.9%、39.4%和8.7%,对照组分布为45.1%、45.1%和9.7%。各位点基因型分布比较,差异均无统计学意义(P>0.05)。进一步按年龄分层分析显示,≤50岁和>50岁不同年龄组各基因型分布在对照组和宫颈癌组中比较,差异无统计学意义(P>0.05)。结论 mi R-423 rs6505162、mi R-205 rs3842530和mi R-1307 rs7911488基因多态性可能与宫颈癌发生无关。
Objective To investigate the relationship between gene polymorphism of miR-423,miR-205 and miR-1 307 with cervical cancer risk. Methods A case-control study was carried out including 104 patients with cervical cancer and 186 controls such as fracture and hypertension during the same period. Mi R-423 rs6505162 polymorphism was examined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) assay. The gene polymorphism of miR-205 rs3842530 and miR-1307 rs7911488 were detected by multiple PCR and multiple LDR method. The association between the genotypes and cervical cancer was analyzed by Chi-square test. Results The genotype of rs6505162 were 64.4% for CC, 31.7% for AC, 3.8% for AA in cases and 66.1% for CC, 30.1% for AC, 3.8% for AA in controls. The genotype of rs3842530 were 56.7% for-/-, 34.6%for-/insert, 8.7%for inser/inser in cases and 52.7% for-/-, 36.0% for-/insert, 7.0% for inser/inser in controls. The genotype of rs7911488 were 51.9% for AA, 39.4% for AG, 8.7% for GG in cases and 45.1% for AA, 45.1%for AG, 9.7% for GG in controls. The genotype distribution of miR-423, miR-205 and miR-1307 polymorphism in the cases and the controls had no statistically significant difference(P〉0.05). Further agestratified analysis showed that these three polymorphism genotype frequencies distribution had no statistical significance between ≤ 50 years old and 〉50 years old in the cases and the controls(P〉0.05). Conclusion There are no association between gene polymorphism of miR-423 rs6505162, miR-205 rs3842530 and miR-1307 rs7911488 and cervical cancer.
作者
王春桃
冯蓉
张艳秋
申娴
隋静
梁戈玉
WANG Chuntao FENG Rong ZHANG Yanqiu SHEN Xian SUI Jing LIANG Geyu(College of Nursing, Jiangsu Vocational College of Medicine, Jiangsu 224001, China)
出处
《中国妇产科临床杂志》
CSCD
北大核心
2017年第4期337-340,共4页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
国家自然科学基金(81472939
81673132)
盐城市医学科技发展计划项目(YK2016055
YK2013060)
江苏省示范性高等职业院校建设项目(SJG201519)