秦皇岛市山海关区副溶血弧菌污染分布及耐药性

Pollution distribution and drug resistance of Vibrio parahaemolyticus in Shanhaiguan District of Qinhuangdao City

目的通过对水产品、外环境和腹泻便中副溶血弧菌的分离鉴定,掌握副溶血弧菌的污染分布状况,有效预警食源性疾病的发生,同时对分离出的副溶血弧菌进行耐药性分析,为临床用药提供科学依据。方法水产品按GB 4789.7-2013检验,外环境参照海水中副溶血弧菌检验方法的优化第一法,腹泻便检验依据WS 271-2007附录B,分离鉴定出副溶血弧菌,并对分离出的副溶血弧菌进行耐药性试验。结果水产品、外环境和腹泻便中副溶血弧菌检出率分别为21.72%、1.61%和12.38%,其中水产品中鱼、虾、蟹、贝和软体动物的检出率分别为16.56%、27.27%、20.41%、29.46%和10.00%。外环境中水体、泥沙、水生植物和水生动物检出率分别为2.16%、1.71%、0.76%和1.33%。腹泻便中检出率为12.38%。结论山海关区腹泻便中副溶血弧菌耐药日趋严重,推荐环丙沙星和左氧氟沙星等喹诺酮类抗生素作为治疗副溶血弧菌感染的首选药物。

[Objective]To grasp the pollution distribution of Vibrio parahaemolyticus via the isolation and identification of it in aquatic products,environment and diarrhea stool,to conduct effective early warning of foodborne disease,to perform drug resistance analysis of isolated strains,and provide scientific basis for clinical use. [Methods] Aquatic products were tested according to GB 4789.7-2013,external environment was tested according to the first optimization test method of parahemolytic vibrio in seawater. Diarrhea stool was tested according to WS 271-2007 appendix B. The isolated strain of Vibrio parahaemolyticus was conducted drug resistance testing. [Results] The detection rate of Vibrio parahaemolyticus in aquatic products,environment and diarrhea was 21.72%,1.61% and 12.38%,respectively. The detection rate of Vibrio parahaemolyticus in fish,shrimp,crabs,shellfish and mollusks was 16.56%,27.27%,20.41%,29.46% and 10.00%,respectively. The detection rates of water,sediment,aquatic plants and aquatic animals were 2.16%,1.71%,0.76% and 1.33% respectively in the external environment,that of diarrhea stool was 12.38%. [Conclusion]Vibrio parahaemolyticus resistance is becoming more and more serious in Shanhaiguan District. Ciprofloxacin and levofloxacin are recommended as the preferred drug for the treatment of Vibrio parahaemolyticus infection.