二代测序在胰腺癌中的研究进展

Progress of Next-generation Sequencing in Pancreatic Cancer Research

胰腺癌是高度恶性的消化系统肿瘤。由于极差的预后及有限的治疗方法,对胰腺癌分子生物学更进一步的了解及研究,可能会明显提高诊断的精确性及治疗的有效性。二代测序(next-generation sequencing,NGS)是对传统测序一次革命性的改变,具有高通量、高敏感性、高自动化程度等优点。利用二代测序技术,筛选胰腺癌致病基因,研究胰腺癌的发病机制,进而指导胰腺癌的诊断及治疗,有望改进胰腺癌的诊疗现状。近年来,二代测序在胰腺癌的应用中取得巨大进展,该文就二代测序及其在胰腺癌中的研究进展作一综述。

Pancreatic cancer is a highly malignant tumor of digestive system. Due to the poor prog- nosis and limited treatment methods,the further understanding and research on molecular biology of pancreatic cancer,may improve the accuracy of diagnosis and the effectiveness of treatment significantly. Next-generation sequencing （NGS） has revolutionized the traditional sequencing pro- cess with the characteristic of high throughput,high sensitivity,and high degree of automation. Using NGS technology in screening related genes to research the pathogenesis of pancreatic eaneer, is expected to improve the status of diagnosis and treatment of pancreatie eaneer. In reeent years, the application of NGS in panereatie eaneer has made great progress;this article intends to review these progress.