氧化石墨烯装载白藜芦醇用于多种肺腺癌细胞的体内外治疗

Preparation of graphene oxide loaded with resveratrol for in vitro and in vivo therapy of several human lung adenocarcinoma cells

目的:制备装载白藜芦醇(Resveratrol,RV)的氧化石墨烯(Graphene Oxide,GO)纳米颗粒并用于人肺腺癌细胞的体内外治疗研究。方法:紫外分光光度计检测GO装载RV前后的紫外吸收光谱;原子力显微镜和纳米粒度分析仪检测纳米颗粒的表面形态和粒径大小;细胞活性检测试剂盒CCK-8检测纳米颗粒对A549细胞活性的影响;最后构建小鼠肿瘤模型,并研究纳米药物对肿瘤的治疗效果。结果:制备GO装载的RV颗粒(NGO-RV)为片层纳米结构,平均粒径大小为98 nm。在p H为5.0时,RV的释放量达到最大。NGO-RV对A549、NCI-H358细胞的抑制作用具有浓度和时间依赖性,明显比单独的RV细胞抑制作用大。在体治疗实验发现,NGO-RV具有显著的A549肿瘤抑制作用。结论:本实验成功制备的NGO-RV药物,在体内外的肿瘤治疗中发挥显著效果。

Objective To prepare the graphene oxide（GO） loaded with resveratrol（RV）,namely nanoparticle GO-RV（NGORV）,for in vitro and in vivo therapy of human lung adenocarcinoma cells.Methods UV-VIS spectrophotometer was used to test the absorbance spectra of GO before and after being loaded with RV.Atomic force microscopy and Naosizer were used to detect the morphology and diameter of the prepared NGO-RV.The effects of NGO-RV on the A549 cell viability were tested with the cell counting kit-8.Finally,we established the mouse tumor model for evaluating the therapeutic effect of prepared NGO-RV for tumor in vivo.Results The prepared NGO-RV contained nanostructure（lamellar） and about 98 nm in average diameter,and the RV release amount reached maximum when the p H value was 5.0.Compared with RV,NGO-RV showed a significantly larger inhibition on the cell viability of A549 and NCI-H358 cells,with concentration and time dependence.In the in vivo experiment,NGO-RV exhibited obvious A549 tumor suppression.Conclusion We successfully prepare NGO-RV which is significant for in vitro and in vivo therapy of tumor.