肠舒颗粒对腹泻型肠易激综合征大鼠血VIP、SP的影响

Effects of Changshu granules on serum VIP and SP levels in rats with irritable bowel syndrome of diarrhea type

目的:探讨肠舒颗粒对腹泻型肠易激综合征(IBS)大鼠血中血管活性肠肽(VIP)、P物质(SP)的影响。方法:将40只大鼠随机分为空白组、模型组、肠舒颗粒组、氯化钠注射液组、模型组。采用"精神刺激+饮食失宜+劳倦"方法建立肝郁脾虚型IBS动物模型。模型建立后,空白组、模型组给予正常喂养,肠舒颗粒组与氯化钠注射液组分别给予相应药物灌胃。采用酶联免疫法测定血中VIP、SP水平。结果:模型组VIP、SP水平高于空白组、肠舒颗粒组(P<0.05或P<0.01);肠舒颗粒组与空白组比较,差异无统计学意义(P>0.05);但与氯化钠注射液组比较,差异有统计学意义(P<0.05)。结论:肠舒颗粒通过减少胃肠激素分泌释放而抑制IBS(腹泻型)的肠运动功能,为临床治疗腹泻型IBS提供了实验依据。

Objective：To investigate the effects of Changshu granules on serum levels of vasoactive intestinal peptide （VIP） and substance P（SP） in rats with irritable bowel syndrome （IBS）of diarrhea type. Methods：A total of 40 rats were equally and randomly divided into blank group,model group,Changshu granule group ,and control（ sodium chloride injection） group. A rat model of IBS with liver depression and spleen deficiency was established by ＂mental stimulation ＋ improper diet ＋ overstrain＂. After the establishment of model, the blank group and the model group were given normal feed- ing, while the Changshu granule group and the control group were given Changshu granules and sodium chloride injection, respectively, by gavage. Enzyme - linked immunosorbent assay was used to determine serum VIP and SP levels. Results： The model group had significantly higher serum VIP and SP levels than the blank group and the Changshu granule group （P 〈 0. 05orP 〈0.01 ）. There were no significant differences in serum VIP and SP levels between the Changshu granule group and the blank group （ P 〉 0.05 ）. However, there were significant differences in serum VIP and SP levels between the Changshu granule group and the control group（P 〈 0. 05）. Conclusion：For rats with IBS of diarrhea type, Changshu granules can inhibit gastrointestinal motility by reducing gastrointestinal hormone levels to provide a reference for the treatment of IBS of diarrhea type.