宫颈癌组织中P21 mRNA的表达及临床意义

The expression and clinical significance of P21 mRNA in cervical cancer tissue

目的研究P21在宫颈癌和相应癌旁组织中的表达以及与临床病理参数之间的关系。方法收集2010年1月—2016年2月巴彦淖尔市医院就诊的子宫颈癌患者40例,从宫颈癌组织及相应癌旁组织中提取RNA后反转录成c DNA,以GAPDH作为内参,利用PT-PCR技术对P21基因的表达情况进行分析计量资料采用t检验,P<0.05为差异有统计学意义。结果宫颈癌组织和相应癌旁组织中P21 mRNA对GAPDH的相对丰度值分别是(0.16±0.26)和(0.30±0.28),两组表达差异有统计学意义(P<0.05),并且宫颈癌组织中P21 mRNA表达水平与病理分化程度、国际妇产科联盟(international federation of gynecology and obstetrics,FIGO)分期、淋巴结转移、浸润深度有关,与组织学类型和年龄无关。结论 P21在宫颈癌组织中低表达,可能与宫颈癌的发生发展密切相关。

Objective To study the expression of P21 in cervical carcinoma and corresponding adjacent tissues as well as the relationship between clinicopathological parameters.Methods The total RNAs were extracted from 40 pairs of cervical carcinoma tissue and corresponding adjacent tissues from January 2010 to February 2016 and the RNAs were reverse transcripted into c DNA.The PT-PCR was used to analyse the expression of P21 with GAPDH as internal control.Measurement data was processed by t test,the result of P 0.05 was considered statistically significant.Results The relative abundance of P21 mRNA to GAPDH mRNA in cervical cancer and adjacent tissue were（0.16±0.26） and（0.30±0.28） respectively and there was statistically significant difference between them（P0.05）.The expression of P21 mRNA may be associated with pathological grade,FIGO stage,lymph node metastasis,depth of invasion,regardless of histological type and age.Conclusion The expression of P21 may play an important role in the occurring of cervical carcinoma tissue.