生脉注射液对大鼠放射性肺炎及血清细胞因子IL-6、TNF-α、TGF-β_1表达的影响

Effects of Shengmai injection on radiation pneumonitis and serum cytokines IL-6,TNF-α,TGF-β_1 expression

目的为临床应用生脉注射液防治放射性肺炎提供实验依据。方法选择2016年8-10月SPF级雄性SD大鼠40只,随机分为正常对照组、模型组、生脉注射液组和地塞米松组,每组10只。利用6 mv X线,对全胸进行单次照射,总剂量为20 Gy,建立放射性肺炎大鼠模型。除正常对照组不进行胸部放射照射外,其余三组均进行胸部放射照射。生脉注射液组灌胃输入生脉注射液0.6 ml/100 g体质量,地塞米松组进行灌胃输入地塞米松注射液0.05 mg/100g体质量,正常对照组和模型组给予同体积生理盐水灌胃。照射后第1天开始用药,1次/d,连续灌胃至照射后满6周处死动物。观察各组大鼠肺大体改变并计算肺系数;HE染色观察肺组织病理变化;ELISA法检测血清白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、转化生长因子-β_1(transforming growth factor-beta1,TGF-β_1)水平。计量资料采用单因素方差分析,每两组之间用t检验,P<0.05为差异有统计学意义。结果生脉注射液组肺系数为(0.331±0.020),血清IL-6为(258.45±9.05)ng/L,TNF-α为(317.34±17.36)ng/L,TGF-β_1为(263.81±14.17)ng/L;地塞米松组肺系数为(0.363±0.013),血清IL-6为(351.87±12.12)ng/L,TNF-α为(391.07±13.83)ng/L,TGF-β_1为(368.25±21.26)ng/L,模型组肺系数为(0.398±0.015),血清IL-6为(615.29±20.21)ng/L,TNF-α为(738.15±23.52)ng/L,TGF-β_1为(619.64±19.43)ng/L。上述各项指标生脉注射液组及地塞米松组均显著低于模型组,对比差异均有统计学意义(均P<0.05),且上述各项指标生脉注射液组均优于地塞米松组,对比差异均有统计学意义(均P<0.05)。结论生脉注射液可能通过抑制血清中IL-6、TNF-α、TGF-β_1的合成,从而对大鼠放射性肺炎起到防治作用。

Objective It is made to provide experimental evidence for the clinical application of Shengmai injection in preventing radiation pneumonitis.Methods Forty male Sprague-Dawley（SD） rats were randomly divided into normal control group,model group,Shengmai injection group and dexamethasone group from August to October 2016.10 cases in each group.The 6 mv X line of 20 Gy irradiated the full chest of these rats by single irradiation,which established the rat model of pneumonia.In addition to the normal control group without radiation of chest irradiation,the rest of the three groups needed the chest irradiation.Shengmai injection group was given 0.6 ml/100 g body weight of the drug solution and dexamethasone group 0.05 mg/100 g body weight of it.Normal control group and model group were given the same volume of normal saline.After irradiation,these animals started to be used medication,once a day and continuously fed and irradiated at least six weeks,after which these animals were sacrificed.Observe the change of the rat lung substantially and calculate the lung coefficient,pathological changes in lung tissue staining HE,ELISA assay serum interleukin-6（IL-6）,tumor necrosis factor-α（TNF-α） and the transforming growth factor-β1（TGF-β1） levels.The data were analyzed by one-way ANOVA,and the t-test was used between the two groups.When the difference was P0.05 which means value was analyzed statistically.Results The pulmonary coefficient of Shengmai injection group was（0.331±0.020）,serum IL-6 was（258.45±9.05） ng/L,TNF-α was（317.34±17.36） ng/L,TGF-β1 was（263.81±14.17） ng/L,dexamethasone group the level of lung tissue was（0.363±0.013）,serum IL-6 was（351.87±12.12） ng/L,TNF-α was（391.07±13.83） ng/L,TGF-β1 was（368.25±21.26） ng/L,the model group the level of lung tissue was（0.398±0.015）,IL-6 was（615.29±20.21） ng/L,TNF-α was（738.15±23.52） ng/L and TGF-β1 was（619.64±19.43） ng/L,and the above parameters were significantly better than those in the dexamethasone group（all P0.05）,and the above indexes were significantly lower than those in the model group（all P0.05）.Conclusion Shengmai injection may play a preventive effect on radiation pneumonitis in rats by inhibiting the synthesis of serum of IL-6,TNF-α and TGF-β1.