槟榔花茶对大鼠亚急性酒精性肝损伤的保护作用

Protective Effects of Areca Inflorescence Tea on Alcohol-induced Hepatic Injury in Rats

本文研究了槟榔花茶对亚急性酒精性肝损伤是否有辅助保护作用。50只SD雌性大鼠,随机分成5组(乙醇模型对照组、溶剂对照组和低、中、高剂量组)。剂量组经口灌胃受试物,而溶剂对照组和乙醇模型组灌胃蒸馏水。乙醇模型组和低、中、高剂量组持续30 d灌胃受试物4 h后给予10 m L/kg体重的50%乙醇,而溶剂对照组给予等量蒸馏水。然后对血清中ALT、AST、CHO、TBIL、LDL含量进行检测,并取肝脏进行组织病理学检查。结果显示:随槟榔花茶剂量增加,样品各剂量组血清中AST、ALT、CHO、TBIL和LDL值呈下降趋势,其中高剂量组与乙醇模型组间存在显著差异(p<0.05)。组织病理学检查显示:高剂量组能改善肝细胞脂肪变性,且肝脏病理总分显著低于模型对照组(p<0.05)。综上,槟榔花茶对亚急性酒精性肝损伤有辅助保护功能。

The auxiliary protective effects of areca inflorescence tea on acute alcoholic liver injury were investigated in this study. Fifty SD female rats were randomly divided into five groups, including an ethanol model（control） group, a solvent control group, and low-, medium-, and high-dose areca tea groups. Areca tea was administered by oral gavage to each dose group, while the solvent control group and the ethanol model group were given by gavage. Four hours after gavage, the ethanol model group, and the low-, medium-, and high-dose groups were given 50% ethanol at a dose of 10 m L/kg weight for 30 d, while the solvent control group was given an equal volume of distilled water. The content of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, cholesterol（CHO）, total bilirubin（TBIL）, and low-density lipoprotein（LDL） in serum were then measured, and histopathological examination of liver tissues was also performed. The results showed that the content of AST, ALT, CHO, TBIL, and LDL in the serum were decreased with increasing doses of areca tea, and significant differences were observed between the high dose group and the ethanol model group（p〈0.05）. The histopathological examination of liver tissues revealed that liver steatosis was mitigated in the high dose group, and the liver pathological scores of the high dose group were significantly lower than those of the model control group（p〈0.05）. In conclusion, areca inflorescence tea can exert an auxiliary protective effect against acute alcoholic liver injury.