玫瑰细胞露的化学成分及活性研究

Phytochemical Constituents, and Antioxidant, Antimicrobial, and Tyrosinase Inhibitory Activities of Rose Oil Distillation Wastewater

玫瑰细胞露是玫瑰花在水蒸气提取玫瑰精油后产生的玫瑰花水溶液。本文针对玫瑰细胞露进行了植物化学分析和生物活性分析,通过GC-MS定量分析结合HPLC分析发现玫瑰细胞露主要化学成分包括:芳樟醇,苯乙醇,香茅醇,α-红没药醇,山奈酚-3-O-芦丁苷,同时对细胞露的酪氨酸酶抑制活性,抗氧化活性,抗菌活性进行了系统评价。结果表明:细胞露的酪氨酸酶抑制活性为IC50=2.45 m L,DPPH抗氧化活性为IC50=2.58 m L,ABTS自由基清除活性为3.43 m L;另外,细胞露具有较强的抗菌活性,对具核梭杆菌有较强的抑制率,最低抑菌浓度为MIC=32μL,最低杀菌浓度MBC=125μL。为玫瑰细胞露在化妆品和食品行业中的应用提供理论基础。

Rose oil distillation wastewater（RODW） is the waste solution that obtained after the preparation of rose essential oil by water vapor extraction of roses. The phytochemical constituents of RODW and their biological activities were investigated in this study. Gas chromatography-mass spectrometry（GC-MS） quantitative analysis combined with high-performance liquid chromatography（HPLC） showed that the main chemical components of RODW included linalool, phenethyl alcohol, citronellol, α-bisabolol, and kaempferol-3-O-rutinoside, and the antioxidant, antimicrobial, and tyrosinase inhibitory activities of RODW were systematically evaluated. The results showed that the half maximal inhibitory concentration（IC50） values for tyrosinase inhibitory activity, 2,2-diphenyl-1-picrylhydrazyl（DPPH） radical scavenging activity, and 2,2′-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid（ABTS） radical scavenging activity of RODW were 2.45±0.14 m L, 2.58±0.04 m L, and 3.425±0.21 m L, respectively. Furthermore, RODW possessed strong antimicrobial activity, and showed a high inhibition rate against Fusobacterium nucleatum,with a minimum inhibitory concentration（MIC） of 32 μL and minimum bactericidal concentration（MBC） of 125 μL. This study provides a theoretical basis for the application of RODW in cosmetics and food industries.