薏苡叶水提液对小鼠抗疲劳与耐缺氧作用的实验研究

Experimental study of Coix lacryma-jobi (L.) leaf 's water extract on the anti-fatigue and hypoxia tolerance function in mice

目的研究薏苡叶水提液对小鼠抗疲劳、耐缺氧作用。方法按体重将雄性小鼠平均分为4组:对照组、低中高3个剂量实验组。3个剂量实验组分别给予1.5,4.5,7.5 g·kg^(-1)·d-1的薏苡叶水提液,对照组灌以蒸馏水,连续灌胃14d。进行负重游泳和常压耐缺氧实验,测定游泳时间和死亡时间,并称量肝重,计算肝重系数。结果高中低3个剂量实验组小鼠负重游泳时间分别为6.74,10.47,5.51 min;高中低3个剂量实验组小鼠常压耐缺氧时间分别为46.48,54.96,54.90 min;高中低3个剂量实验组小鼠肝重系数分别为0.054,0.050,0.051。与对照组比较,中剂量实验组小鼠负重游泳时间差异有统计学意义(P<0.05);中低2个剂量实验组小鼠常压耐缺氧时间差异均有统计学意义(均P<0.01);低中高3个剂量实验组小鼠肝重系数差异均有统计学意义(均P<0.05)。结论薏苡叶水提液对小鼠有抗疲劳、耐缺氧作用。

Objective To study the anti-fatigue and hypoxia tolerance function of Coix lacryma-jobi leaf＇ s water extract in mice. Methods Normal male mice were averagely selected and divided into four groups by weight： control group,low dose,medium dose and high dose experimental groups. Coix lacryma-jobi leaf＇ s water extract of different dose（ 1. 5,4. 5,7. 5 g · kg^（-1）） was given into the mice＇ s stomach respectively in three experimental groups and distilled water was given in control group,every day for a continuous 14 days. Burdened swimming test and hypoxia tolerance test were conducted in mice to determine their swimming time and death time. Dissociating the mouse＇s liver,weighing the mouse＇s liver and calculating the liver weight coefficient. Results The burdened swimming time of the high dose group,medium dose group and low dose group were respectively 6. 74,10. 47,5. 51 min. The hypoxia tolerance time of the high dose group,medium dose group and low dose group were respectively 46. 48,54. 96,54. 90 min. The liver weight coefficient of the high dose group,medium dose group and low dose group were respectively0. 054,0. 050,0. 051. Compared with the control group,the burdened swimming time of the medium dose group was longer than that of the control group with significantly（ P〈0. 05）. The death time of the low dose group and the medium dose group were prolonged than that of the controlgroup with significantly（ all P〈0. 01）. Weighing the mouse＇s liver and the liver weight coefficient in three experimental groups were all higher than the control group with significantly（ all P〈0. 05）. Conclusion Coix lacryma-jobi leaf＇s water extract had anti-fatigue and hypoxia tolerance function in mice.