姜黄素对人食管癌耐药细胞Eca-109/VCR生长和凋亡的影响

Effect of curcumin on proliferation and apoptosis of human esophageal carcinoma drug-resistant cells Eca-109/VCR

目的研究姜黄素(Cur)对人食管癌耐药细胞Eca-109/VCR的体内外生长和凋亡的影响。方法 CCK-8法测定Cur对Eca-109/VCR细胞的抑制作用。FCM检测Eca-109/VCR细胞凋亡率。建立Eca-109/VCR裸鼠皮下移植瘤模型,观察Cur对移植瘤的抑制作用。比较各组瘤组织病理形态学改变、细胞凋亡及血清中Caspase-3、Caspase-8、Caspase-9蛋白的表达。结果 Cur呈浓度、时间依赖性抑制Eca-109/VCR细胞增殖。Cur可诱导Eca-109/VCR细胞凋亡。Cur显著抑制移植瘤的生长,Cur组内可见大量坏死灶。Cur诱导移植瘤细胞凋亡和升高Caspase-3、Caspase-8、Caspase-9的表达,随着Cur浓度的增高而增加。结论Cur能显著抑制食管癌耐药细胞的体内外生长,其作用可能与上调Caspase-3、Caspase-8和Caspase-9表达,诱导耐药癌细胞凋亡有关。

Objective To investigate the effect of curcumin （Cur） on proliferation and apoptosis of human esophageal carcinoma drug-resistant ceils Eca-109/VCR in vitro and in vivo. Methods The inhibitory effect of Cur on Eca-109/VCR was detected by CCK-8 method. The apoptosis rate of Eca-109/VCR ceils after treatment with Cur was determined by flow cytometry. Eca-109/VCR xenografts were established in nude mice and inhibitory effect of Cur on xenografts was observed. HE staining was used to observe the morphological changes. Apoptosis was detected by TUNEL. ELISA was used to measure Caspase-3, Caspase-8 and Caspase-9 expression in nude mice serum. Results Cur significantly inhibited the proliferation of Eca- 109/VCR in a time and concentration-dependent manner and it could induce apoptosis of Eca- 109/VCR. Cur significantly inhibited the growth of xenografts and a large number of necrosis existed in Cur group. Cur induced apoptosis in xenografts and the expression of Caspase-3, Caspase-8 and Caspase-9 in serum increased with the increase of Cur concentrations. Conclusion Cur could inhibit the growth of esophageal carcinoma xenografts in vitro and in vivo and its role might be up-regulating the expression of Caspase-3, Caspase-8 and Caspase-9 and associated with the apoptosis induction of drug-resistant esophageal carcinoma cells.