喉癌与HPV、EBV感染阳性表达的相关性

Correlation of positive expressions of HPV and EBV with laryngeal carcinoma

目的探讨喉癌与HPV、EBV阳性表达的关系。方法采用核酸分子快速导流杂交基因芯片分型技术(Hybri Max)和实时荧光定量PCR技术对101例喉癌石蜡包埋组织标本进行37种HPV亚型检测和EBV定量检测,以43例声带息肉标本为对照。结果喉癌组HPV阳性率13.86%,对照组HPV阳性率9.3%,HPV在喉癌组和对照组的阳性表达差异无统计学意义(P>0.05);喉癌组EBV阳性率63.37%,对照组EBV阳性率13.95%,EBV在喉癌组和对照组的阳性表达差异具有统计学意义(P<0.05);通过不同分化程度喉癌组织中EBV阳性率的比较,EBV在高分化型与中分化型喉癌组织中阳性表达差异无统计学意义(P>0.05),EBV在高分化型与低分化型、中分化型与低分化型喉癌组织中阳性表达差异具有统计学意义(P<0.05);不同性别、年龄、病程的喉癌患者中EBV阳性率差异无统计学意义(P>0.05)。结论喉癌的发病与EBV感染密切相关,与HPV可能有关,HPV高危亚型感染在诱发喉癌的过程中有一定作用;喉癌患者的性别、年龄、病程长短与EBV感染无明显关系。本研究将为喉癌的病毒学致病机制及临床上防治喉癌提供一定的依据。

Objective To investigate the relationships between expressions of HPV and EBV in laryngeal carcinoma. Methods DNA flow-through hybridization and gene chip genotyping technology （HybriMax） and real-time quantitative PCR were used for 37 subtypes of HPV detection and quantitative detection of EBV in 101 cases of laryngeal cancer paraffin embedded tissue specimens. 43 cases of vocal cord polyp of paraffin embedded tissue specimens were used as the controls. Results The positive rate of laryngeal carcinoma was 13.86% in group HPV and 9.3% in the control group, with no statistically significant difference between the positive expressions of HPV in the laryngeal carcinoma group and control group （P 〉 0.05 ）. The positive rate of laryngeal carcinoma was 63.37% and 13.95%, respectively, in group EBV, and the control group, with significant difference between them （P 〈 0.05 ）. In respect of the positive rate by comparing differently differentiated EBV in laryngeal carcinoma, there was no significant difference in the positive expression of EBV in well differentiated and differentiated laryngeal carcinoma （P 〉 0.05 ） , but the difference was statistically significant in highly differentiated EBV as compared with those with low differentiation type, medium differentiation and poor differentiation （P 〈 0.05 ）. There was no significant difference between the groups in view of sex, age and course of disease in the patients （P 〉 0.05 ）. Conclusions The incidence of laryngeal carcinoma is closely related with EBV infection, possibly relationed with HPV and high-risk subtypes of HPV have a certain role in the process of induced laryngeal carcinoma. The gender, age and duration of disease have no significant correlation with EBV infection. This study will provide a basis for further invesgitation of pathogenesis of laryngeal cancer and prevention and treatment of laryngeal cancer.