miR-499A>G基因多态性与汉族人群大肠癌发病及预后关联分析

Association of miR-499A>G polymorphism and incidence and prognosis of colorectal cancer in Han Chinese

目的了解miR-499A>G与广西壮族自治区汉族人群大肠癌发病及预后关系。方法TaqMan探针检测miR-499A>G基因型分布,Logistic回归模型及Cox风险回归模型分析其与大肠癌发病及预后关联。结果共收集病例对照394对,多因素Logistic回归分析显示,相加模型下miR-499A>G并未增加大肠癌发病风险(OR=1.22,95%CI:0.95~1.57)。携带AA、AG、GG基因型大肠癌患者中位生存时间分别为56、43和18个月,Log Rank检验其分布差异有统计学意义(F=11.24,P=0.004);COX风险回归模型分析miR-499A>G(HR=1.26,95%CI:1.02~1.57)与不良预后显著相关。结论 miR-499A>G与汉族人群大肠癌发病无关联但影响其预后。

Objective To explore the relationship between polymorphism of miR-499A 〉 G with incidence and prognosis of colorectal cancer in Han Chinese. Method 1 ： 1 matched case control study was used to collect samples, and the patients were followed up. The genotypes distribution miR-499A 〉 G were detected by TaqMan probe, and logistic regression model and Cox proportional hazard regression model were used to analysis the relationship between miR-499A 〉 G and incidence and prognosis of coloreetal cancer. Results 394 cases and 394 controls were recruited, while miR-499A 〉 G did not increase the risk of colorectal cancer （ OR = 1.22, 95% CI： 0.95-1.57） under additive model analyzed by multi-logistic regression model. The means of survival time of AA, AG, GG carriers were 54.19± 2.02, 44.16 ± 2.59,32.76± 5.36 months and there were significant differentiation between them in log Rank test （F = 11.24, P= 0.004）. Cox proportional hazard regression model showed that miR-499A 〉 G was significant associated with adverse prognosis of colorectal cancer （HR= 1.26, 95%CI： 1.02- 1.57）. Conclusion miR-499 A 〉 G has no association with the incidence of coloreetal cancer, but it affects the prognosis of colorectal cancer in Han population.