分光光度法测定香肠中亚硝酸盐含量的不确定度评定

Uncertainty Evaluation of Nitrite Content in Sausage Detected by Spectrophotometry

评定分光光度法测定香肠中亚硝酸盐含量的不确定度。根据GB/T 5009.33-2010中亚硝酸盐的测定原理和方法,建立相应的数学模型,运用测量不确定度评定的基本方法,对模型中各影响因素的不确定度进行计算和评定。结果表明:香肠样品液中亚硝酸盐的质量引入的不确定度最大,是合成不确定度的主要来源。其中亚硝酸盐曲线拟合引入的不确定度U_(relsam)(C)是各分量中引入不确定度最大的一项;当取置信概率为95%时,最终得到测量的扩展不确定度为0.095mg/kg(k=2)。该方法对分光光度法测定香肠中亚硝酸盐含量结果的不确定度评定具有参考作用。

Evaluate the uncertainty of nitrite content in sausage detected by spectrophotometry. Mathematical model is established on the basis of the principle and method of nitrite determination in GB/T 5009. 33- 2010. The uncertainties of factors in mathematical model are analyzed and calculated. The results show that the uncertainty of nitrite in the quality is the largest in sausage sample liquid and it is the main source of compound uncertainty. Urelsam （C） is the main source of the component uncertainties, which is synthesized and extended. An expanded uncertainty of 0. 095 mg/kg is obtained at the confidence probability of 95% and coverage factor of 2. The method provides reference for the uncertainty evaluation of nitrite content in sausage detected by spectrophotometry.