慢性HBV感染者GPI、MMP-1、MMP-3测定的临床意义

The clinical significance of the detection of GPI, MMP-1,MMP-3 in patients with chronic HBV infection

目的:检测葡萄糖-6-磷酸异构酶(glucose-6-phosphate isomerase,GPI)、基质金属蛋白酶(matrix metalloproteinase,MMP)-1、MMP-3血清标志物在慢性乙型肝炎(chronic hepatitis B,CHB)、肝硬化(liver cirrhosis,LC)及肝细胞肝癌(hepatocellular carcinoma,HCC)患者中的表达水平,探讨其在慢性肝病及肝癌进展过程中的变化及意义。方法:应用酶联免疫吸附试验定量检测受检者血清中GPI、MMP-1、MMP-3的浓度,AU2700全自动生化分析仪测定谷丙转氨酶(alanine aminotransferase,ALT)及谷草转氨酶(aspartate transaminase,AST),分析GPI、MMP-1、MMP-3与ALT、AST的相关性。其中CHB 31例,LC 40例、HCC 46例,健康体检者50例。结果:3组肝病患者中血清GPI、MMP-1、MMP-3表达水平均显著高于健康对照组(均P<0.05);在LC患者中,随着AST的增加MMP-3浓度增高,二者呈弱正相关(r=0.18,P<0.05)。AST>40 IU/L LC患者血清MMP-3浓度显著高于AST≤40 IU/L者(P<0.05)。GPI、MMP-1与ALT、AST均无相关性。结论:血清GPI、MMP-1、MMP-3可作为乙型肝炎病毒感染相关肝病肝损伤的诊断指标。

Objective： To evaluate the levels of three serum marker as glucose-6-phosphate isomerase （GPI）, matrix metall- oproteinase（MMP）-1, MMP-3 in patients with chronic hepatitis B（CHB）, liver cirrhosis（LC） and hepatocellular carcinoma （HCC）, for researching the changes and the significance of the process in chronic liver disease and liver cancer. Methods： The concentration of serum GPI, MMP-1, MMP-3 in 31 patients with chronic hepatitis B viral hepatitis were examined by enzyme-linked immunoabsorbent assay（ELISA）, the change of alanine aminotransferase（ALT） and aspartate transaminase（AST） by AU2700 automatic biochemical analyzer. Further to analyse the relationship among three kinds of serum markers and ALT and AST in peripheral blood. 31 patients with CHB, 40 patients with LC, 46 patients with HCC and 50 normal control. Results： The concentration of serum GPI, MMP-1, MMP-3 in the three kinds of liver disease are obviously higher than that of healthy controls, P values are less than 0.05; the concentration of MMP-3 is increased along with AST in patients with liver cirrhosis, there is weak positive correlation between them（r=0.18, P〈0.05）. MMP-3 in patients with liver cirrhosis that AST〉 40 IU/L are significantly higher than that AST≤40 IU/L（P〈0.05）. There is no correlation between GPI, MMP-1 and ALT, AST. Conclusion： The levels of GPI, MMP-1, MMP-3 can be used as the diagnostic index of hepatitis B virus related liver damage.