用于促黄体生成素快速检测的电化学免疫传感器

An Electrochemical Immunosensor for Rapid Detection of Luteinizing Hormone

研制了一种用于促黄体生成素(LH)检测的电化学免疫传感器,实现了促黄体生成素的高灵敏度和快速检测需求。采用纸基免疫传感器作为基底材料,氨基化石墨烯(NH2-G)/硫堇/纳米金复合物修饰的丝网印刷工作电极(SPWE)作为检测系统的工作电极。该免疫传感器的检测原理是:电化学物质硫堇通过电极表面氧化还原反应产生电化学电流,抗原抗体的特异性结合会影响电化学电流的释放,导致电流下降,并且检测电流与抗原的浓度呈反比。实验结果表明:对于促黄体生成素在1~100 mIU·mL^(-1)内该传感器具有很好的检测结果,检测的最低限为1 mIU·mL^(-1),检测的线性系数为0.991,并且检测时间可以控制在10 min。因此该传感器可用于促黄体生成素的快速和高灵敏度检测需求,这对于性激素水平的动态监测,进而实现不孕不育疾病个性化诊断治疗以及指导备孕都有着重要的意义。

An electrochemical immunosensor for detection of luteinizing hormone（LH）antigen was developed to realize the high sensitive and rapid detection.With the paper-based immunosensor as the base material,the screen-printed working electrode（SPWE）was modified by the amino functional graphene（NH2-G）/thionine/gold nanoparticles.The detection principle of the immunosensor is that the electrochemical material thionine produces the electrochemical current through the redox reaction of the electrode surface,and the specific binding of the antigen antibody will affect the release of the electrochemical current to decrease the current.The detect current is inversely proportional to the concentration of the antigen.The experimental results show that the sensor has good test results for luteinizing hormone（LH）in the range of 1-100 mIU· mL-1,the lowest limits is 1 mIU·mL-1,the linear coefficients is 0.991,and the detection time can be controlled within 10 min.Therefore,the immunosensor can be used for rapid and high sensitivity detection of luteinizing hormone.Thus,it has an important significance to dynamically monitor the sex hormone levels,then realize personalized diagnosis and treatment of infertility disease and guide the preparation for pregnancy.