氨基化荧光碳点制备及其荧光探针构建

Preparation and evaluation of fluorescent carbon dots(CDs) and CDs based immunofluorescence probe

目的 :依据抗原-抗体免疫荧光分析原理,构建荧光碳点-大肠杆菌抗体免疫荧光探针。方法 :选取富含氨基的氨基葡萄糖为碳源,通过微波加热法制备荧光碳点,再通过EDC/NHS化学偶联法与大肠杆菌抗体结合,形成免疫荧光探针。利用荧光光谱仪、透射电子显微镜、红外光谱仪、紫外-可见光吸收光谱仪等评价碳点以及荧光探针的理化性能。结果:制备的碳点具有优异荧光性能与水溶性,构建的免疫荧光探针可实现大肠杆菌快速检测。结论:碳点是一种环境友好、荧光性能稳定的新型纳米功能材料,有望成为一种优秀的检测试剂。

Objective To fabricate an immunofluorescence probe system of carbon dots conjugated antibody based on antigen-antibody reaction principles. Methods A green one-step microwave assisted pyrolysis method was applied to preparing fluorescent carbon dots（CDs） using aminoglucose as carbon source and the obtained CDs were conjugated with antibody via EDC/NHS reactions to build CDs based fluorescent probe. Furthermore, the properties of CDs and CDs based probe system were evaluated by Fourier transform infrared（FTIR） spectra, transmission electron microscopy（TEM）, UV-vis absorption and so on. Results The as-prepared CDs showed excellent fluorescence and hydrophilicity and CDs based immunofluorescence probe exhibited the capability of quick detection of E.coli O157∶ H7. Conclusion Fluorescent CDs as one new emerging environment-friendly nanomaterial has great potential in biosensors.