miRNA在重度子痫前期胎盘组织中的表达水平

The expression of miRNA in placenta in severe preeclampsia

目的探讨微小RNA(mi RNA)在重度子痫前期胎盘组织中的表达水平,为重度子痫患者的治疗机制提供基础。方法选取本院2014年12月-2015年12月收治的重度子痫前期患者8例作为研究,同时选取同期健康体检孕妇者6例进行对比,通过荧光定量PCR检测技术检测胎盘组织中mi RNA-125a-3p、mi RNA-1290以及mi RNA-133b等3个具有代表的mi RNA指标,观察两组表达水平的差异。结果重度子痫患者胎盘组织中mi RNA-125a-3p、mi RNA-1290以及mi RNA-133b表达水平显著高于健康孕妇,差异有统计学意义,P<0.05。结论重度子痫前期患者胎盘组织中mi RNA的表达水平显著高于正常孕妇,其可能参与了重度子痫前期的发病及病理生理过程,说明胎盘组织中mi RNA有可能成为一种新的标记物用于重度子痫的早期诊断。

Objective： To investigate the micro RNA （miRNA） expression levels in severe preeclampsia placenta, provide the basis for the treatment of patients with severe preeclampsia mechanism. Methods： selected in our hospital in December 2014 and 2015 December severe preeclampsia patients 8 cases as the research. At the same time, the selection of healthy pregnant women during the same period in 6 cases were compared by fluorescence quantitative PCR detection technology in placenta tissues was detected miRNA-125a-3p, miRNA-1290 and miRNA-133b etc. three is representative of miRNA targets, to observe the differences of the expression level of two groups. Results： The expression of miRNA-125a-3p, miRNA-1290 and miRNA- 133b in placenta of severe preeclampsia patients was significantly higher than that in healthy pregnant women, the difference was statistically significant, P〈0.05. Conclusion： Severe expression levels of miRNAs in placenta of patients with preeclampsia was significantly higher than that of normal pregnant women, which may be involved in the severe preeclampsia pathogenesis and pathophysiological process that miRNA in placental tissue has the potential to become a new marker for severe preeclampsia of early diagnosis.