摘要
在CIK细胞的培养过程中加入卡介菌多糖核酸,研究其对CIK细胞体外增殖及杀瘤活性等方面的影响.CCK-8检测结果发现低浓度卡介菌多糖核酸处理的CIK细胞相比对照组(生理盐水),细胞增殖能力和杀瘤效果都显著上调.RT-qPCR结果显示卡介菌多糖核酸处理后的CIK细胞毒活性相关因子(Granzyme B,IFN-γ,TNF-α和TNF-β)mRNA的表达相比对照组都有提高.这些结果可为培养具有更高效杀瘤活性又能大量扩增的CIK细胞提供新思路.
The BCG polysaccharide and nucleic acid （BCG-PSN） was added to CIK ceils to study CIK cells＇ responses on the proliferation and tumoricidal activity in vitro. CCK-8 results showed that the CIK cells treated with low dose BCG-PSN, compared with control group （normal saline）, were significantly enhanced on the proliferation and tumoricidal activity. RT-qPCR results revealed that the expression of CIK Granzyme B, IFN-T, TNF-α, TNF- β mRNA of BCG-PSN group was higher than that of the control group. These results lay the foundation for how to cultivate a large number of CIK cells with high tumoricidal activity, and provide new ideas for the therapy of anti- tumor adoptive immunity.
作者
黄招琴
苏壬香
钟贝芬
韩慧敏
谷陟欣
颜冬兰
辛秀
袁莉
胡翔
HUANG Zhao-qin SU Ren-xiang ZHONG Bei-fen HAN Hui-min GU Zhi-xin YAN Dong-lan XIN Xiu YUAN Li HU Xiang(Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China Jiuzhitang Limited Share Company Postdoctoral Workstation, Changsha 410205, China)
出处
《湖南师范大学自然科学学报》
CAS
北大核心
2017年第2期33-38,共6页
Journal of Natural Science of Hunan Normal University
基金
湖南省博士后基金资助项目(2012RS4016)
长沙市科技局重点资助项目(K1303113-31)
