miR-145在非小细胞肺癌中的表达

The expression of miR-145 in non-small cell lung cancer

目的:检测非小细胞肺癌患者癌血清和组织中miR-145的表达量与临床病理参数的关系,探讨其作为非小细胞肺癌分子标志物的可能性。方法:采用实时荧光定量PCR检测非小细胞肺癌及健康血清,以及非小细胞肺癌组织和对应癌旁组织中miR-145的表达量,分析miR-145的表达与非小细胞肺癌临床病理参数的关系。采用ROC曲线分析miR-145对非小细胞肺癌的诊断价值。结果:miR-145在癌组织中的表达量明显低于癌旁组织(P<0.05);miR-145在患者血清中的表达显著低于对照组(P<0.05)。miR-145的ROC曲线下面积(AUC)为0.875,灵敏度为88.5%,特异度为84.8%。结论:非小细胞肺癌患者癌组织和血清miR-145低表达,miR-145有望作为NSCLC早期的诊断指标。

Objective： To investigate the expression of miR-145 in non-small cell lung cancer（ NSCLC） and explore it＇s potential as a biomarker for non-small cell lung cancer. Methods： Real time-PCR was used to detect the expression of miR-145 in serum of normal human and patients with non-small cell lung cancer and in cancer tissues and their corresponding nontumor tissues. The diagnostic value of miR-145 for non-small cell lung cancer was assessed by ROC curve. Results： The expression level of serum miR-145 in patients with non-small cell lung cancer was significantly lower than that of normal human（ P 〈0. 05）. The expression of miR-145 in non-small cell lung cancer was much lower than in the adjacent normal tissues（ P 〈0. 05）. The area under ROC curve of miR-145 was 0. 875,and the sensitivity and specificity were 88. 5% and 84. 8%. Conclusion： The low expression of miR-145 in cancer tissues and serum from non-small cell lung cancer patients,may be used as noninvasive biomarker for diagnosis of non-small cell lung cancer.