摘要
目的:观察内质网应激蛋白GRP78在宫颈癌组织中的表达情况,探讨宫颈癌组织中HPV感染状态与GRP78表达的关系。方法:收集手术切除的宫颈癌组织标本89例,分析高危型HPV感染和低危型HPV感染与患者年龄、病理分化、临床分期及淋巴转移的关系,用免疫组化法检测GRP78在不同HPV亚型感染的宫颈癌组织中的表达情况。结果:在宫颈癌组织中,HPV(+)感染率在低分化鳞癌中高于高分化鳞癌(P<0.05),宫颈癌II期中高于宫颈癌I期(P<0.05),有淋巴结转移的患者中高于无淋巴结转移的患者(P<0.05);GRP78蛋白在高危型HPV感染的宫颈癌组织中阳性表达率显著高于低危型HPV感染及HPV阴性的宫颈癌组织(P<0.01),而GRP78在低危型HPV感染及HPV阴性的宫颈癌组织中表达无明显差异(P>0.05)。结论:HPV(+)感染率随宫颈癌恶性程度增高而增加,高危型HPV感染的宫颈癌组织中GRP78蛋白呈高表达。
Objective: To observe expression of endoplasmic reticulum stress protein GRP78 in cervi- cal cancers and discuss the possible relationship between HPV infection status and GRP78 in cervical cancers. Methods: 89 cervical cancer tissue specimens were obtained from surgical resection in de- partment of Obstetrics and Gynecology, and the statistical method was also adopted to analyze the cor- relations between HPV infection status in cervical cancer tissues and patients" indexes involving age, pathological differentiation, clinical stage and lymph node metastasis, respectively. Then, the expres- sion of GRP78 in cervical cancer tissues infected with different HPV subtypes was detected by immuno- histochemical method. Results: In clinical cervical cancer tissues, the infection rate of HPV ( + ) in cervical cancer tissues were higher in poorly differentiated squamous carcinoma than those in high dif- ferentiated squamous carcinoma ( P 〈 0.05 ). The infection rate of HPV ( + ) in stage II cervical canc- er tissues were higher than in stage I cervical cancer tissues ( P 〈 0.05 ). The infection rate of HPV ( + ) in cervical cancer tissues with lymph node metastasis were higher than those without lymph node metastasis in cervical cancer specimens ( P 〈 0.05 ). Immunohistochemistry showed that the positive expression rate of GRP78 in high-risk HPV-infected cervical cancer tissues was significantly higher than that in low-risk HPV-infected and HPV-negative cervical cancer tissues (P 〈0.01 ), and the ex-pressions of GRP78 in low-risk HPV-infected and HPV-negative cervical cancer tissues had no signifi- cant difference (P 〉 0.05 ). Conclusions: HPV ( + ) infection was positively correlated with the degree of malignancy of cervical cancer. GRP78 protein was related to the high-risk HPV infection.
作者
刘卿
汪俊涛
秦娟
郭兵
LIU Qing WANG Juntao QIN Juan GUO BING(Department of Pathology and Physiology, Guizhou Medical University, Guiyang 550004, Guizhou, China Guiyang Maternal and Child Health-Care Hospital, Guiyang 550004, Guizhou, China)
出处
《贵州医科大学学报》
CAS
2017年第7期763-766,771,共5页
Journal of Guizhou Medical University
基金
国家自然科学基金资助项目(NS2015-36)
贵州省科学技术基金[黔科合LH(2014)7297号]
贵阳市卫生局科学技术计划资助项目[(2013)筑卫科技合同字第创21号]
关键词
宫颈肿瘤
人乳头瘤病毒
内质网应激
葡萄糖调节蛋白78
cervical tumor
human papilloma virus
endoplasmic reticulum stress
glucose regulatory protein 78