内质网应激蛋白GRP78在宫颈癌组织中的表达及意义

Expression and Significance of Endoplasmic Reticulum Stress Protein GRP78 in Cervical Cancer Tissues

目的:观察内质网应激蛋白GRP78在宫颈癌组织中的表达情况,探讨宫颈癌组织中HPV感染状态与GRP78表达的关系。方法:收集手术切除的宫颈癌组织标本89例,分析高危型HPV感染和低危型HPV感染与患者年龄、病理分化、临床分期及淋巴转移的关系,用免疫组化法检测GRP78在不同HPV亚型感染的宫颈癌组织中的表达情况。结果:在宫颈癌组织中,HPV(+)感染率在低分化鳞癌中高于高分化鳞癌(P<0.05),宫颈癌II期中高于宫颈癌I期(P<0.05),有淋巴结转移的患者中高于无淋巴结转移的患者(P<0.05);GRP78蛋白在高危型HPV感染的宫颈癌组织中阳性表达率显著高于低危型HPV感染及HPV阴性的宫颈癌组织(P<0.01),而GRP78在低危型HPV感染及HPV阴性的宫颈癌组织中表达无明显差异(P>0.05)。结论:HPV(+)感染率随宫颈癌恶性程度增高而增加,高危型HPV感染的宫颈癌组织中GRP78蛋白呈高表达。

Objective： To observe expression of endoplasmic reticulum stress protein GRP78 in cervi- cal cancers and discuss the possible relationship between HPV infection status and GRP78 in cervical cancers. Methods： 89 cervical cancer tissue specimens were obtained from surgical resection in de- partment of Obstetrics and Gynecology, and the statistical method was also adopted to analyze the cor- relations between HPV infection status in cervical cancer tissues and patients＂ indexes involving age, pathological differentiation, clinical stage and lymph node metastasis, respectively. Then, the expres- sion of GRP78 in cervical cancer tissues infected with different HPV subtypes was detected by immuno- histochemical method. Results： In clinical cervical cancer tissues, the infection rate of HPV （ ＋ ） in cervical cancer tissues were higher in poorly differentiated squamous carcinoma than those in high dif- ferentiated squamous carcinoma （ P 〈 0.05 ）. The infection rate of HPV （ ＋ ） in stage II cervical canc- er tissues were higher than in stage I cervical cancer tissues （ P 〈 0.05 ）. The infection rate of HPV （ ＋ ） in cervical cancer tissues with lymph node metastasis were higher than those without lymph node metastasis in cervical cancer specimens （ P 〈 0.05 ）. Immunohistochemistry showed that the positive expression rate of GRP78 in high-risk HPV-infected cervical cancer tissues was significantly higher than that in low-risk HPV-infected and HPV-negative cervical cancer tissues （P 〈0.01 ）, and the ex-pressions of GRP78 in low-risk HPV-infected and HPV-negative cervical cancer tissues had no signifi- cant difference （P 〉 0.05 ）. Conclusions： HPV （ ＋ ） infection was positively correlated with the degree of malignancy of cervical cancer. GRP78 protein was related to the high-risk HPV infection.