脉络膜脱离型孔源性视网膜脱离玻璃体液中前列腺素水平的研究

Prostaglandin levels in vitreous of patients with Rhegmatogenous retinal detachment associated with choroidal detachment

目的验证前列腺素E2(PGE2)、前列腺素F2α(PGF2α)作为脉络膜脱离型视网膜脱离的特征性分子标志物的意义。方法收集2014年9月—2015年9月南京医科大学附属无锡第二人民医院眼科的56例原发性病例,包括26例脉络膜脱离型孔源性视网膜脱离(RRDCD)患者及30例孔源性视网膜脱离(RRD)患者,对玻璃体液分别进行前列腺素E2、F2α酶联免疫吸附试验(ELISA)分析。结果 RRDCD组玻璃体液中PGE2含量低于RRD组,组间差异具有统计学意义(P<0.01),而RRDCD组玻璃体液中PGF2α与RRD组相比差异无统计学意义(P=0.865)。RRDCD患者不同PVR等级(A、B、C、D)玻璃体液中PGE2、PGF2α浓度,差异均无统计学意义(P=0.409、0.389)。将RRDCD患者轻度PVR(A、B)合并后与严重PVR(C、D)比较,玻璃体液的PGE2、PGF2α浓度水平差异均无统计学意义(P=0.433、0.948)。结论 RRDCD患者玻璃体液中PGE2水平低于RRD组,与前期研究结果一致,可能是PGE2在诱发炎症的过程中消耗过多,或者是限制非特异性炎症的应答丧失,导致RRDCD的炎症程度较RRD严重,且PGE2并不随PVR的严重程度而发生变化。而PGF2α在本次研究中没有表现出具有RRDCD分子标志物的潜能。

Objective To verified the changes of prostaglandin E2 （PGE2） and prostaglandin F2 （PGF2α） as potential molecular markers in rhegmatogenous retinal detachment（RRD） associated with choroidal detachment（CD）. Methods Total 56 primary patients （26 cases of RRD associated with CD and 30 cases of RRD） undergoing pars plana vitrectomy in Nanjing Medical University Affiliated Wuxi Second Hospital between September,2014 and October,2015 were collect- ed. The level of prostaglandin E2 and F2α in vitreous fluid were tested by using ELISA. Results PGE2 level in vitreous of RRD associated with CD group was lower than that in RRD group, the difference was statistically significant （ P 〈 0.01 ）. However, the PGF2α level was not significantly difference between the two groups（P = 0. 865）. In the RRD asso- ciated with CD group ,the level of PGE2 and PGF2α in vitreous had no significant difference between different grades of PVR（ A, B, C, D）,P = 0. 409,0. 389;the level of PGE2 and PGF2α had no significant difference between mild PVR（ A and B） and heavy PVR（ C and D） , P = 0. 433 ,0. 948. Conclusion The PGE2 level in vitreous of RRD associated with CD was less than RRD and this resuh is consistent with earlier study. It might indicate a greater consumption of PGE2 that would be stimulated by inflammation or a loss of limitation of inflammatory responses. Besides, the level of PGE2 is not correlated with PVR grades. PGF2α does not show the potential as biomarker in process of RRDCD.