大孔树脂纯化蓝莓果渣花青素的研究

Study on Purification of Anthocyanin in Blueberry Pomace by Macroporous Resins

目的:筛选分离纯化蓝莓果渣花青素的最佳树脂,优化纯化蓝莓果渣花青素的工艺条件。方法:比较AB-8、D101两种大孔树脂对蓝莓果渣花青素的吸附解吸效果,研究了AB-8大孔树脂对蓝莓果渣花青素的吸附解吸条件。结果:AB-8大孔树脂是纯化蓝莓果渣花青素较好树脂,其对蓝莓果渣花青素吸附解吸平衡时间均为4 h;吸附最适温度60℃、样液稀释4倍、p H 3.0、吸附流速1 m L/min时吸附能力最佳,乙醇浓度60%、p H 3.0、解吸流速1 m L/min时解吸能力最佳。结论:该工艺得到的花青素产品为紫黑色粉末,色价为16.90,是未纯化样品色价的5倍。

Objective： The purpose of this study is to screen the best macroporous resin on purification of anthocyanin in blueberry pomace and optimize the purification of anthocyanin in blueberry process conditions. Methods：The adsorption and desorption effects of AB-8,D101 macroporous resins to blueberry pomace anthocyanins were compared. The adsorption and desorption properties of resin AB-8 on anthocyanin from blueberry pomace were further inwestigated. Results： It was found that AB-8 was the best resin on purification from blueberry pomace anthocyanin and the time of adsorption and desorption equilibrium was 4 h,At the conditions of temperature 60 ℃,liquid sample dilution 4 times,p H 3. 0,velocity of adsorption was 1 m L/min,the highest adsorption ability for bluebrry pomace anthocyanin was achieved. At the conditions of desorption solution concentration 60%,p H 3. 0,velocity of desorption was 1 m L/min which could provide the best desorption effect on anthocyanin from AB-8. Conclusion： By combination of those optimal condition,a purplish dark powder with colour value16. 90,was that of unnatural purified sample 5 times.