细菌纤维素/聚谷氨酸复合水凝胶的辐照制备及性能

Preparation of Bacterial Cellulose/Poly γ-Glutamic Acid Composite Hydrogels via Co^(60) γ-Irradiation Crosslinking and Their Properties

采用Co^(60)-γ射线辐照交联法制备细菌纤维素/聚谷氨酸(BC/PGA)复合水凝胶。采用红外光谱和扫描电子显微镜等对复合水凝胶的结构进行表征,研究了BC引入对复合水凝胶的凝胶分数、热失重、溶胀性能、压缩性能和流变性能的影响,并利用CCK-8法对复合水凝胶进行了细胞毒性评价。研究结果表明,辐照作用下BC纳米纤维和PGA形成双交联复合凝胶网络,BC可有效增加复合水凝胶的压缩强度、储能模量(G')和凝胶分数,降低复合水凝胶的平衡溶胀度。50kGy辐照剂量下,相对于纯PGA水凝胶,复合水凝胶压缩强度增大5倍,G'增大10倍。同时复合水凝胶无细胞毒性,可安全应用于生物医学领域。

The bacterial cellulose/poly γ-glutamic acid （BC/PGA） composite hydrogels were prepared by Co60 γ- irradiation crosslinking method. The composite hydrogels were characterized by infrared spectra （IR） and scanning electron microscope （SEM）, and their gel fraction, thermal stability, swelling behavior, compression properties and theological properties were investigated. Additionally, their cytotoxicity was evaluated by CCK-8 assay. The results show that BC nanofibers and PGA can form the composite hydrogels with double crosslinking structure via γ- irradiation. The addition of BC increases compressive strength, storage modulus （G＇） and the gel fraction but decreases the equilibrium swelling ratio of the BC/PGA composite hydrogels. The compressive strength and storage modulus of composite hydrogels increase 5 times and 10 times respectively at the irradiation dose of 50 kGy. Moreover, the BC/PGA composite hydrogels are non-toxic, indicating their safety for biomedical application.