CSN5在胃癌中的表达及与患者预后的相关性

Expression of CSN5 and its correlation with the prognosis of gastric carcinoma

目的探讨CSN5在胃癌中的表达及与患者预后的关系。方法运用cBioportal分析工具,分析CSN5在胃癌中基因水平的改变情况;运用qRT-PCR方法检测CSN5 mRNA在永生化的正常胃黏膜细胞株及不同胃癌细胞株中的表达;运用Oncomine数据库(DErrico、Chen胃癌数据集)和TCGA数据库(375例胃癌组织和32例癌旁组织),分析CSN5 mRNA在胃癌组织与正常胃黏膜表达的差异性;运用生存期分析软件Kaplan-Meier plotter,分析CSN5对876例胃癌患者预后的影响;运用Chen胃癌数据集,分析胃癌患者5年生存状态与CSN5表达的关系。结果在胃癌中CSN5主要的基因改变形式为扩增;CSN5 mRNA在多种胃癌细胞株中的表达水平显著高于正常胃黏膜细胞株;CSN5 mRNA在胃癌中的表达显著高于正常胃黏膜。生存分析结果发现,CSN5高表达的患者总生存期较长,CSN5低表达的患者总生存期较短。结论 CSN5在胃癌组织中高表达,且其表达水平与胃癌患者的预后呈正相关,即CSN5高表达的患者,预后较好。

Objective To investigate the expression of CSN5 in gastric carcinoma （GC） and to determine the prognos- tic value of CSN5 for GC patients. Methods The genomic alternation of CSN5 was assessed using cBioportal analyti- cal tool. CSN5 mRNA expressions in normal gastric cell line and GC cell line were determined using qRT-PCR. The differences of CSN5 mRNA expressions in gastric mucosa and GC tissues were analyzed based on Oncomine database and TCGA database. The association between CSN5 and prognosis of GC patients was determined with Kaplan-Meier plotter. The relationship between CSN5 expression and 5-year survival of GC patients was analyzed using Chen gastric statistics. Results CSN5 was mainly amplified in GC. The mRNA expression of CSN5 was significantly higher in canc- er cell lines than in normal gastric cell line, and was significantly higher in GC tissues than in normal gastric mucosa. Pa- tients with high expression of CSN5 had longer overall survival （OS） than those with low expression. Conclusion The expression of CSN5 is elevated in GC tissues, which is also positively correlated with OS of GC patients.