lncRNA AK093987在结肠癌组织中的表达及其临床意义

Expression of lncRNA AK093987 in colon carcinoma tissue and its clinical significance

目的:探讨长链非编码核糖核酸AK093987(lnc RNA AK093987)在结肠癌组织中的表达及其临床意义。方法:收集2011年1月至2015年12月四川内江市第一人民医院收治的65例结肠癌患者的肿瘤组织及癌旁组织和15例先天性巨结肠和各种原因结肠破裂穿孔、肠扭转、嵌顿疝患者的正常结肠组织。通过Real-time PCR法检测lnc RNA AK093987在65例结肠癌组织、癌旁正常组织及结肠癌细胞中的表达,CCK8法检测转染si RNAAK093987下调lnc RNA AK093987表达的结肠癌Lo Vo细胞的增殖。采用Chi-Square检验和Kaplan-Meier法分别分析lnc RNA AK093987表达与临床病理参数、生存时间和预后的关系。Cox回归分析影响结肠癌患者DFS和OS的因素。结果:lnc RNA AK093987在结肠癌组织中的表达明显高于癌旁组织和正常结肠组织(7.125±1.398 vs 1.058±0.070、1.092±0.049,均P<0.01)。下调lnc RNA AK093987表达的结肠癌Lo Vo细胞增殖显著降低(P<0.05)。lnc RNA AK093987的表达与疾病分期、淋巴结转移、远处转移、肿瘤分化、血清CEA水平和生存状态明显相关(均P<0.05);而与患者的性别、年龄及肿瘤部位无显著相关(均P>0.05);高表达lnc RNA AK093987患者的中位DFS和OS均较低表达者明显缩短[DFS:(13.00±1.49)vs(27.01±1.87)个月;OS:(27.00±3.32)vs(43.72±3.08)个月,均P<0.01];lnc RNA AK093987的表达、远处转移及临床分期是结肠癌独立的预后因素(P<0.05)。此外,下调lnc RNA AK093987的表达能够明显抑制肿瘤细胞的增殖。结论:lnc RNA AK093987参与结肠癌的发生发展,lnc RNA AK093987的表达与疾病分期、淋巴结转移、远处转移、肿瘤分化、血清CEA水平和患者生存状态有关,可作为潜在的结肠癌诊断和预后评估的分子标志物。

Objective： To explore expression of long strand non-coding ribonucleic acid （lonRNA） AK093987 in colon carcinoma tissues and its clinical significance. Methods： Cancer and para-cancer tissues of the 65 patients with carcinoma of colon who hospitalized in the 1 st People’s Hospital of Neijiang, Sichuan, during January 2011 to December 2015, as well as normal colon tissues of the 15 patients with congenital dilatation of colon, and perforation of colon, colonal twist and incarcerated hernia caused by various reasons were collected. Expressions of lncRNAAK093987 in cancer and para-cancer tissues of the 65 patients with carcinoma of colon and the colon cancer cells were detected by RT-PCR assay. Proliferation of the colon cancer LoVo cells that were transfected with siRNA AK093987 to down-regulate expression of lncRNAAK093987 was tested by CCK8 assay. Cji-Square test and Ka-plan-Meier assay were used respectively to analyze association of expression of lncRNA AK093987 with clinical features, survival and prognosis of the patients with carcinoma of colon. The factors affecting DFS and OS of the patients with carcinoma of colon were analyzed by Cox regression analysis. Results： Expression of lncRNA AK093987 in the cancer tissues of colon was obviously higher than those in the paracancer tissue and the normal colon tissues （7.125±1.398 vs 1.058±0.070 and 1.092±0.049, all P〈0.01）. Proliferation of the LoVo cells down-regu-lating expression of lncRNA AK093987 significantly reduced （P〈0.05）. Expression of lncRNA AK093987 was re-markably related to clinical staging, metastasis of lymph node, distant metastasis, differentiation of tumor, serum CEA level and survival status of the patients with carcinoma of colon （all P〈0.05）. DFS and OS medians of the patients with high expression of lncRNA AK093987 were significantly shortened than those in the patients with low expression of lncRNAAK093987 （DFS： [13.00±1.49] months vs [27.01±1.87] months; OS： [27.00±3.32] months vs [43.72±3.08] months, all P〈0.01）. Expression of lncRNA AK093987, distant metastasis and clinical staging were the independent prognostic factors of the patients with colon cancer （P〈0.05）. In addition, down-regulation of ln-cRNA AK093987 did evidently inhibit proliferation of the colon cancer LoVo cells. Conclusion： lncRNA AK093987 might involve in development of the colon cancer. Expression of lncRNAAK093987 could be obviously correlated to clinical staging, metastasis of lymph node, distant metastasis, differentiation of tumor, serum CEA level and survival status of the patients with colon cancer, which might be a potential molecular marker for diagnosis and prognosis assessment of the colon carcinoma.