一测多评法定量分析灵芝及灵芝孢子粉中单体三萜成分的应用

Simultaneous Determination of Ganoderic Acid in Sporophore and Spore of Ganoderma by Quantitative Analysis of Multi-Components by Single Marker

目的:建立灵芝及灵芝孢子粉中灵芝酸A、灵芝酸C_2的一测多评含量测定方法。方法:采用高效液相色谱(HPLC)法,以灵芝酸A为内标物,建立其与灵芝酸C_2的相对校正因子,并进行含量测定,实现一测多评。采用外标法测定10批灵芝及灵芝孢子粉中灵芝酸C_2含量验证一测多评法的准确性。结果:采用外标法、一测多评法测得灵芝酸C_2含量的平均相对误差为1.07%,含量计算值与实测值无统计学差异(P>0.05)。结论:建立的校正因子在不同实验条件下重现性良好,在对照品缺乏的情况下,以灵芝酸A为内参物建立灵芝孢子粉中2种指标成分含量测定的一测多评法可行。

Objective：To establish a method for determination of ganoderic acid A and ganoderic acid C2 in sporophore and spore of ganoderma by quantitative analysis of multi-components by single marker （QAMS）. Methods： The relative correction factors of ganoderic acid C2 were determined by HPLC,while using ganoderic acid A as the internal standard substance. The contents of ganoderic acid C2 in 10 samples of sporphore and spore of ganoderma were authentically determined by the external standard method, so as to verify the accuracy of QAMS method. Results： There was no significant difference hetween the external standard method and QAMS,the average relative error of ganoderic acid C2 was 1.07% by two methods. Conclusion：The established RCFs have good repeatability in different experimental conditions. In case of lack of standard substances, the QAMS method with ganoderic acid A as an internal standard for determination of ganoderic acid in sporophore and spore of ganoderma is feasible.