miR-15b在恶性黑色素瘤A375细胞中的表达及其对细胞转移和侵袭的影响

Expression of MIR-15B in Malignant Melanoma and Its Effects on Cell Migration and Invasion

目的研究微小RNA-15b(microRNA-15b,miR-15b)在恶性黑色素瘤A375细胞中的表达,以及miR-15b对恶性黑色素瘤细胞转移和侵袭能力的影响。方法应用荧光实时定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)方法检测恶性黑色素瘤A375细胞与正常黑素细胞HM中miR-15b的表达水平。沉默恶性黑色素瘤A375细胞中miR-15b的表达后,应用划痕实验检测miR-15b对恶性黑色素瘤A375细胞迁移距离的影响,应用Transwell实验检测miR-15b对恶性黑色素瘤A375细胞转移和侵袭能力的影响。结果 miR-15b恶性黑色素瘤A375细胞中的表达水平显著高于正常黑素细胞。沉默恶性黑色素瘤细胞中miR-15b的表达后,细胞迁移距离、细胞转移和侵袭能力均受到显著抑制。结论miR-15b在恶性黑色素瘤A375细胞中表达显著升高并可促进恶性黑色素瘤A375细胞转移和侵袭。

Objective To investigate the expression of miR - 15b in malignant melanoma cell line A375 ,and further investigate the effects of miR - 15b on cell migration and invasion of A375 cells. Methods The expression of miR - 15b in A375 cells and human mela- nocyte cell lines HM was detected by qRT- PCR. After silencing the expression of miR - 15b in A375 cells, we detected the effects of miR - 15b on cell migrating distance by wound healing assays, we also detected the effects of miR - 15b on cell migration and invasion a- bility by TranswelI assays. Results The expression of miR - 15b was significantly upreglated in A375 cells than HM cells. After silen- cing the expression of miR - 15b in A375 cells, the cell migrating distance, cell migration and cell invasion was significantly inhibited. Conclusion miR - 15B was upregnlated in malignant melanoma cell line A375, and could promote the migration and invasion of A375 cells.