摘要
目的探讨特发性卵巢早衰与AMH,AMHR-Ⅱ的基因多态性。方法选择2015年6月~2017年3月在我院诊断的特发性卵巢早衰患者50例为POF组。另选择健康体检者100例为对照组。PCR方法测定两组AMH,AMHR-Ⅱ基因多态性。结果 POF组AMH基因突变位点基因型及等位基因频率与对照组比较差异无统计学意义(P>0.05)。POF组AMHR-Ⅱc.49+10T>G基因位点GG基因型比例显著高于对照组,G等位基因频率显著高于对照组,c.622-2C>T基因位点TT基因型比例显著高于对照组,T等位基因频率显著高于对照组,c.622-24C>A基因位点AA基因型比例显著高于对照组,A等位基因频率显著高于对照组,c.1038G>T基因位点TT基因型比例显著高于对照组,T等位基因频率显著高于对照组,差异均有统计学意义(P<0.05)。结论 AMHR-Ⅱ基因多态性可能是特发性卵巢早衰的重要的发病机制。
Objective To discuss polymorphism analysis of AMH, AMHR-Ⅱ gene in patients with idiopathic premature ovarian failure. Methods 50 cases with idiopathic premature ovarian failure from Jun 2015 to Mar 2017 were selected as POF group. Ang100 cases for physical examination were selected as control group. Polymorphismof AMH, AMHR-Ⅱ gene of two groups was detected by PCR. Results Genotype and allele frequency of AMH gene mutation sites of POF group showed no significant difference with the control group（P 〉 0.05）. The proportion of GG genotype in AMHR- loci -Ⅱ, c.49＋10T 〉 G of POF group was higher than that of the control group, and G allele frequency was higher than that of the Control group; The proportion of TTgenotype inc.622-2C 〉 Tof POF group was higher than that of the control group, and Tallele frequency was higher than that of the control group;The proportion of AAgenotype inc.622-24C 〉 Aof POF group was higher than that of the control group, and Aallele frequency was higher than that of the control group;The proportion of TTgenotype inc. 1038G 〉 Tof POF group was higher than that of the control group, and Tallele frequency was higher than that of the control group; The difference showed significant difference（P 〈 0.05）. Conclusion Polymorphism of AMHR-Ⅱ gene may be an important pathogenesis of idiopathic premature ovarian failure.
出处
《中国医药科学》
2017年第12期7-10,共4页
China Medicine And Pharmacy
基金
广东省公益研究与能力建设专项资金项目(2015A080803009)
广东医科大学科研基金(M2016043)
