SGT基因杂合缺失小鼠血液生理生化指标分析

Analysis of hematological and biochemical parameters of heterozygous SGT-deficient mice in young and elderly ages

目的分析SGT基因杂合缺失对不同年龄小鼠血液指标和代谢的影响。方法分别采集10周龄、26周龄SGT基因杂合缺失(SGT^(+/-))小鼠和野生型小鼠眼眶血液,用全自动血细胞分析仪和生化分析仪检测常用血液生理指标和生化指标,进行不同年龄和基因型间统计比较。结果与同基因型低龄小鼠对比,高龄小鼠多项血液生理生化指标均发生增龄性改变。与同龄野生型对照相比,10周龄SGT^(+/-)小鼠大血小板比率降低,未结合型胆红素浓度降低(P<0.05),其余指标均未见统计学差异;26周龄SGT^(+/-)小鼠血小板计数、血小板比容和中性粒细胞计数降低,碱性磷酸酶、未结合型胆红素、胆固醇及甘油三酯等指标改变有统计学意义(P<0.05)。结论SGT基因杂合缺失能够诱发高龄小鼠部分血液、血清生化指标的改变。

Objective To study the effect of heterozygous deficiency of SGT gene on the hematological and biochemical parameters of mice in young and elderly ages. Methods Blood samples were analyzed for complete hematological and biochemical parameters from heterozygous SGT-deficient and wild-type mice of 10-weeks and 6-months old mice, respectively. Results Age-related changes in most indexes were found statistically significantly different between young and elderly mice with the same genotype. Compared with the wild type at the same age, the platelet large cell ratio （P-LCR） was lower in young heterozygous SGT-deficient mice. However, platelet count, plateletcrit （PCT） and neutrophil count were more significantly lower in elderly heterozygous SGT-deficient mice （ P 〈 0. 05 ）. There was no significant difference for biochemical parameters ALT, AST, LDH, urea nitrogen, creatinine and blood glucose. Total and unconjugated bilirubin as well as ALP were significantly higher in elderly heterozygous SGT-deficient mice but not for conjugated bilirubin （ P 〈 0.05 ）. In addition, significant differences existed for the lipids between two elderly groups （ P 〈 0. 05）. Conclusions Heterozygous deficiency of SGT gene induced changes of some hematological and biochemical parameters in elderly mice. It provides helpful information for further investigation on SGT involvement in some biological and pathological processes.