电针和肾上腺髓质素抗脑缺血再灌注损伤中神经血管效应的初步研究

Preliminary Study of the Neurovascular Effect of Electroacupuncture and Adrenomedullin in Cerebral Ischemia-reperfusion Injury

目的初步揭示电针和肾上腺髓质素(ADM)在抗脑缺血再灌注损伤中的神经血管效应。方法综合运用神经功能缺损评分、体感诱发电位及TTC染色技术,研究大鼠大脑中动脉缺血及再灌注后的改变,以及电针和ADM对其影响。结果电针及ADM可明显改善脑缺血再灌注后神经功能缺损评分(P<0.05),但二者之间差异无统计学意义(P>0.05)。体感诱发电位中P1-N1和N1-P2峰峰值在缺血30 min明显下降(P<0.05),在60 min有所恢复,于再灌注时再次显著降低(P<0.05)。于再灌注后予以电针或ADM处理,可明显改善实验结束时体感诱发电位(P<0.05)。电针和ADM可使脑缺血所致梗死面积明显降低(P<0.05),但二者之间差异无统计学意义(P>0.05)。结论电针可减轻脑缺血再灌注后神经功能损伤,改善脑组织血供,和ADM的神经血管效应有相似之处。

Objective To preliminarily reveal the neurovascular effect of electroacupuncture and adrenomedullin （ADM） in cerebral ischemia-reperfusion injury.Methods Rat changes after middle cerebral artery ischemia and reperfusion, and the effect of electroacupuncture and ADM on them were investigated using the neurological deficit score, somatosensory evoked potentials and TTC staining technique.Results Electroacupuncture and ADM can significantly improve the neurological deficit score after cerebral ischemia and reperfusion （P〈0.05）, but there was no significant difference between the two （P〉0.05）. The P1-N1 and N1-P2 peak values of somatosensory evoked potentials decreased significantly at 30 min after ischemia （P＆lt;0.05）, recovered somewhat at 60 minutes and decreased significantly again during reperfusion （P＆lt;0.05）. Electroacupuncture or ADM during reperfusion could significantly improved somatosensory evoked potentials at the end of experiment （P＆lt;0.05）. Electroacupuncture and ADM could significantly reduce the size of cerebral ischemia-induced infarct （P＆lt;0.05）, but there was no significant differencebetween the two （P〉0.05）.Conclusions Electroacupuncture can reduce neurological impairment and improve brain blood supply after cerebral ischemia and reperfusion. That is similar to the neurovascular effect of ADM.