Bcl-2、PCNA基因在鸡、鹌鹑及其杂交种睾丸组织中的表达研究

Study on Expression of Bcl-2 and PCNA Genes in Testicular Tissue of Chickens,Quails and Chicken-quail Hybrids

为探讨鸡(♂)与鹌鹑(♀)属间杂交种睾丸生长发育不良的分子机理,试验采集新罗曼蛋用型公鸡、朝鲜公鹌鹑、鸡与鹌鹑的雄性杂交种不同发育时期的睾丸组织样共计84份,并用实时荧光定量PCR对公鸡、公鹌鹑及其杂交种各阶段睾丸组织中Bcl-2、PCNA基因的mRNA表达进行了检测。结果显示,鸡和鹌鹑不同生长发育期睾丸组织中Bcl-2、PCNA基因mRNA的表达具有相似性,Bcl-2基因整体呈波动性变化,下降到最低值后,迅速回升到较高水平,PCNA基因均有一个极显著的峰值;与鸡和鹌鹑相比,杂交种Bcl-2、PCNA基因mRNA表达均无明显变化,表明杂交种睾丸发育过程中,Bcl-2基因调控睾丸中细胞凋亡的模式不同于鸡和鹌鹑,也不存在明显的细胞增殖过程,从而推测鸡与鹌鹑的杂交种睾丸生长发育不良的分子影响因素与其睾丸组织中Bcl-2、PCNA基因mRNA的异常表达有关。本试验结果为进一步研究鸡与鹌鹑属间杂交种睾丸发育不良的分子机理提供了参考依据。

In order to investigate the molecular mechanism of stunted growth testis of chicken（♂）and quail（♀）hybrids.84 testicular tissue samples of New Roman cocks,male Korean quails and chicken-quail hybrids at different developmental stages were collected,the mRNA expression of Bcl-2and PCNAgenes in testicular tissue of cocks,quails and chicken-quail hybrids at different growth stages were detected using Real-time PCR.The results showed that the Bcl-2and PCNAgenes mRNA expression patterns in testes of chickens and quails at different growth stages were similar.The Bcl-2gene was fluctuated in a whole,and decreased to the lowest value,then recovered rapidly and remained at a high level.The PCNAgene had a very significant peak value.Compared with the chicken and quail,the expression of Bcl-2and PCNA genes mRNA in hybrids had no obvious change,which indicated that the pattern of the Bcl-2gene regulation apoptosis in testis was different from chicken and quail at the hybrid testicular development process,and there was no obvious cell appreciation process.It was suggested that the molecular influencing factors of testicular dysplasia of chicken and quail hybrids were related to the abnormal expression of Bcl-2and PCNAgenes mRNA in testes.The results provided an important referencefor the further study of the mechanism of testicular dysplasia between chicken and quail interspecific hybrids.