鸡新城疫基因Ⅶ型灭活标记疫苗(MG7株)最小有效免疫剂量的研究

Study on the Minimum Effective Immune Dose of Genotype VII Newcastle Disease Inactivated Marker Vaccine (MG7 strain)

为科学指导鸡新城疫基因Ⅶ型灭活标记疫苗(MG7株)的临床免疫剂量,通过测定免疫鸡HI抗体滴度和攻毒保护试验,进行了10日龄和30日龄SPF鸡最小有效免疫剂量的研究。动物实验使用10μL、20μL和40μL三种不同免疫剂量,免疫疫苗后21 d检测HI抗体效价,10日龄SPF鸡001批次疫苗HI抗体平均效价依次为4.8、6.2和6.5,攻毒保护率依次为80%、100%和100%,30日龄SPF鸡HI抗体平均效价依次为5.0、6.3和6.5,攻毒保护率依次为70%、100%和100%。结果表明,新城疫灭活标记疫苗(MG7株)HI抗体效价和保护效力呈正相关,免疫剂量与HI抗体效价和保护效力也呈正相关。用NDV基因Ⅶ型强毒G7株进行攻毒保护试验,两种日龄鸡在20μL的免疫剂量下,疫苗仍能达到良好的免疫保护效果。

In order to scientifically guide the clinical immunization dose of genotype VII Newcastle disease inactivated marker vaccine （MG7 strain）, the minimum effective immune dose of 10 days and 30 days SPF chickens was studied by measuring the immunized chicken HI antibody and challenge protection test. Animal experiment using 10 μL, 20 μL and 40 μL in three different doses of 3 batches of vaccine immune, The HI antibody titers were detected 21 days after immunization, the 10 day old SPF chickens of 001 batches of vaccine HI antibody titers were： 4.8, 6.2 and 6.5, protective rates were 80%, 100% and 100% .The 30 day old SPF chickens of 001 batches of vaccine HI antibody titers were： 5, 6.3 and 6.5, protective rates were 70%, 100% and 100%.The results showed that there was a positive correlation between the HI antibody titer and protective efficacy after vaccination, and the immunization dose was positively correlated with HI antibody titer and protective effect. The NDV genotype VII virulent G7 strain was used to challenge protection test. The vaccine was able to achieve good immunoprotective effect at 20μL immunization dose.