摘要
研究前列腺素E_2(prostaglandin E_2,PGE_2)在胶原诱导性关节炎(collagen induced arthritis,CIA)小鼠体内的表达,并分析其与滤泡辅助性T细胞(follicular helper T cell,Tfh)之间的相关性。结果显示,与对照组相比,CIA小鼠血清前列腺素E代谢物(prostaglandin E metabolite,PGEM)浓度显著升高,小鼠脾脏和淋巴结中CD4^+CXCR5^+ICOS^+Tfh的比例、CD4^+Bcl-6^+Tfh的比例、CD4^+IL-21^+T细胞的比例均明显增高,且PGEM与上述细胞比例均呈正相关。该研究表明,CIA中PGE_2的表达与Tfh的比例存在正相关性。
To study the expression of prostaglandin E2 （PGE2） and its correlation with follicular helper T cells（Tfh） in mice with collagen-induced arthritis（CIA）, serum levels of prostaglandin E metabolite（PGEM）were detected by ELISA. The per- centages of Tfh in the spleen as well as in the lymph nodes were measured by flow cytometry. Our results showed that PGEM was increased in the sera of mice with CIA. In addition, the percentages of CD4＋ CXCR5＋ ICOS＋ Tfh, CD4＋ Bcl-6＋ Tfh and CD4＋ IL-21＋ T cells were significantly higher than those in the normal controls. Moreover, serum levels of PGEM were posi- tively correlated with Tfh. This study suggests that there is a certain correlation between PGE2 and Tfh in CIA.
出处
《现代免疫学》
CSCD
北大核心
2017年第4期301-307,共7页
Current Immunology
基金
国家自然科学基金(81302569)
上海市科委自然基金青年项目(12ZR14460)
上海市教委科研创新项目(14YZ042)
上海市卫生和计划生育委员会面上项目(20124196
201640137)
上海交通大学医工交叉面上项目(YG2014MS77)
关键词
前列腺素E:
胶原诱导性关节炎
滤泡辅助性T细胞
相关性
prostaglandin E2 （PGE2）
collagen induced arthritis （CIA）
follicular helper T cells （Tfh）
correlation
